Gordonia phage Mocha12
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Detailed Information for Phage Mocha12
Discovery Information
Isolation HostGordonia terrae 3612
Found ByAlexa Sciarra
Year Found2019
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.434509 N, 79.952261 W Map
Discovery NotesThe sample was taken on September 11, 2019 at 8:29 P.M. on Parkview Avenue in South Oakland. The sample was taken around lots of grass and weeds. The soil was very grainy and there were many small pebbles.
Naming NotesMy phage is named Mocha, which was the name of my old childhood dog.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 24, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2034
Genome length (bp)46221
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTACCAGGGGGA
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ2
Cluster Life CycleTemperate
Lysogeny NotesThis phage is most likely producing lysogens. After reviewing both results from my full plate titers, my 10^-4 dilutions both showed no evidence of phage, meaning that it produced a well grown lawn of Gordonia terrae. However, all of the other dilutions I tested created both a webbed plate and plaques decreasing in dilution as the dilutions got smaller.
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesIn the uploaded plaque picture, the plaques are about 1-3 mm in size and appear to be lytic because the plaques are very clear. The phage was incubated at 30°C on PYCa plates with host cell Gordonia terrae.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Mocha12_1
(terminase small subunit)

Mocha12_2
(terminase large subunit)

Mocha12_3
(portal protein)

Mocha12_4
(capsid maturation protease)

Mocha12_5
(scaffolding protein)

Mocha12_6
(major capsid protein)

Mocha12_7

Mocha12_8
(head-to-tail adaptor)

Mocha12_9
(head-to-tail stopper)

Mocha12_10

Mocha12_11
(tail terminator)

Mocha12_12
(major tail protein)

Mocha12_13
(tail assembly chaperone)

Mocha12_14
(tail assembly chaperone)

Mocha12_15
(tape measure protein)

Mocha12_16
(minor tail protein)

Mocha12_17
(minor tail protein)

Mocha12_18
(lysin A)

Mocha12_19

Mocha12_20

Mocha12_21

Mocha12_22

Mocha12_23

Mocha12_24
(lysin B)

Mocha12_25
(minor tail protein)

Mocha12_26

Mocha12_27

Mocha12_28
(minor tail protein)

Mocha12_29

Mocha12_30
(excise)

Mocha12_31

Mocha12_32

Mocha12_33

Mocha12_34

Mocha12_35

Mocha12_36

Mocha12_37
(tyrosine integrase)

Mocha12_38

Mocha12_39
(DNA binding protein)

Mocha12_40
(helix-turn-helix DNA binding domain protein)

Mocha12_41
(metalloprotease)

Mocha12_42

Mocha12_43

Mocha12_44
(immunity repressor)

Mocha12_45
(helix-turn-helix DNA binding domain protein)

Mocha12_46

Mocha12_47
(DNA polymerase III sliding clamp (beta))

Mocha12_48

Mocha12_49

Mocha12_50

Mocha12_51

Mocha12_52

Mocha12_53
(RecE-like exonuclease)

Mocha12_54
(RecT-like ssDNA binding protein)

Mocha12_55

Mocha12_56

Mocha12_57

Mocha12_58

Mocha12_59
(ASC-1 transcription coactivator)

Mocha12_60

Mocha12_61
(DNA methylase)

Mocha12_62

Mocha12_63

Mocha12_64

Mocha12_65

Mocha12_66

Mocha12_67

Mocha12_68
(RusA-like resolvase)

Mocha12_69

Mocha12_70
(helix-turn-helix DNA binding domain protein)

Mocha12_71

Mocha12_72

Mocha12_73

Mocha12_74

Mocha12_75
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW291013
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.33 x 10^10
Pitt Freezer Box# 114
Pitt Freezer Box Grid# G9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload