| Detailed Information for Phage Cynthia |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Haley Shuford |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.445278 N, 79.961111 W Map |
| Discovery Notes | Found at 36 degrees Fahrenheit on 1/26/17 at 12:59 PM. Environmental conditions were mostly cloudy skies with recent precipitation and snow flurries. Collected from soil next to a sidewalk in front of a dormitory building. Soil collection was damp, cold, mud consistency, and medium brown color. Approximately half a cup was obtained. Collected in a plastic bag. Transported for 3 minutes and stored on the surface of a desk at 72.5 degrees Fahrenheit for one and a half hours. |
| Naming Notes | Name came from a popular movie. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 14, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Genome length (bp) | 46219 |
| Character of genome ends | Unknown |
| Overhang Length | 11 bases |
| Overhang Sequence | TACCAGGGGGA |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques of Phage Cynthia are of two different sizes. One larger plaque, approximately one nanometer in size, and one smaller plaque, approximately the size of a pin prick. Both plaque sizes are of clear nature and are circular in shape. |
| Number of Genes | 75 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN586055 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
55 |
| Pitt Freezer Box Grid# |
E5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
