| Detailed Information for Phage DobbysSock |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Gianna Emmett |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.44389 N, 79.9544 W Map |
| Discovery Notes | The soil sample was collected outside of the William Pitt Union from a patch of frozen dirt in a flowerbed. The dirt was hard and uncooperative, so it was difficult to achieve the intended 1/2 cup amount. The sample was frozen and seemed to have a sandy texture until it was thawed while being held in room temperature, where it then became moist. |
| Naming Notes | This phage is a free elf. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 24, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 490 |
| Genome length (bp) | 48198 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCAGGGGGA |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Drexel University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are clear and small in the middle with a medium-sized hazy halo around them, usually oval-shaped. |
| Morphotype | Siphoviridae |
| Number of Genes | 73 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN428048 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
55 |
| Pitt Freezer Box Grid# |
E12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
