| Detailed Information for Phage LordFarquaad |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Former names | Supahhotfire |
| Found By | Christopher DeHaven |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.443472 N, 79.955278 W Map |
| Discovery Notes | I discovered this phage near the WPU patio, under a tree nearby. When the sample was collected it was very wet, as it had rained the night before and was located under a large tree with minimal exposure to sunlight. The sample was dark in color composed mostly of mulch and organic matter. There was no visible sand in the sample. The sample was collected at the surface of the dirt. The ambient temperature was 29 degrees Celsius. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 678 |
| Genome length (bp) | 47863 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TACCAGGGGGA |
| GC Content | 66.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Lord Farquuad plaques were produced by plating a serial dilution of phage particles from a spot test on G. terrae in PYCa and incubated the plate for 5 days. This plate is associated with the 10^-5 dilution. The phage consistently produces plaques which are small (approximately 1-2mm in diameter) round and clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK801734 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
