Detailed Information for Phage Mcklovin |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | McKenna Vettori |
Year Found | 2017 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | 30°C |
GPS Coordinates | 40.446812 N, 79.954169 W Map |
Discovery Notes | Phage adopted from Matthew Weiss; sample found outside Langley Hall at the University of Pittsburgh in a moist flower bed covered in vegetation. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Dec 19, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Approximate Shotgun Coverage | 1881 |
Genome length (bp) | 53155 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGGCTGGGGA |
GC Content | 65.8% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | CZ |
Subcluster | CZ4 |
Cluster Life Cycle | Temperate |
Lysogeny Notes | Evidence towards temperate: plaques appeared cloudy at times. Some high-concentration plates in a serial dilution contained a lawn with few plaques, possibly due to the temperate cycle allowing the bacteria to grow before becoming lytic. |
Other Cluster Members |
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Annotating Institution | Florida International University |
Annotation Status | In GenBank |
Plaque Notes | Plaques are small, toughly 2mm in diameter. Very round, and sometimes ever appear with a cloudy border possibly from temperate life cycle. |
Morphotype | Siphoviridae |
Number of Genes | 77 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Annotation in DNA Master Format | Download |
Submitted Minimal DNA Master File | Download |
Author List | Download |
Annotation Cover Sheet | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MN428055 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
1.0x10^10 pfu/mL |
Pitt Freezer Box# |
57 |
Pitt Freezer Box Grid# |
F11 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |