| Detailed Information for Phage Mcklovin |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | McKenna Vettori |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.446812 N, 79.954169 W Map |
| Discovery Notes | Phage adopted from Matthew Weiss; sample found outside Langley Hall at the University of Pittsburgh in a moist flower bed covered in vegetation. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 19, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1881 |
| Genome length (bp) | 53155 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Evidence towards temperate: plaques appeared cloudy at times. Some high-concentration plates in a serial dilution contained a lawn with few plaques, possibly due to the temperate cycle allowing the bacteria to grow before becoming lytic. |
| Other Cluster Members |
|
| Annotating Institution | Florida International University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small, toughly 2mm in diameter. Very round, and sometimes ever appear with a cloudy border possibly from temperate life cycle. |
| Morphotype | Siphoviridae |
| Number of Genes | 77 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN428055 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.0x10^10 pfu/mL |
| Pitt Freezer Box# |
57 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
