| Detailed Information for Phage MichaelScott |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Jack McAuliffe |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -7°C |
| GPS Coordinates | 40.441944 N, 79.9575 W Map |
| Discovery Notes | I collected the soil sample near a restaurant named Chick'n Bubbly along Oakland Avenue on January 9th, 2017 at 11:12 a.m. The temperature when I collected this was 20 degrees Fahrenheit. It snowed on the previous day, therefore when I collected the sample, there was still a little snow around it. There was a small dead bush near my soil collection spot. The soil sample that I collected was on the surface and was somewhat dry. |
| Naming Notes | Michael Scott is one of the main characters on NBC's television show "The Office." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 25, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 200 |
| Genome length (bp) | 48397 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCAAGGGGA |
| GC Content | 66.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques generated by Phage MichaelScott have small, clear, haloed morphologies. The diameter of the plaques are 1mm in the clear center and 2mm including the halo. When the phage is initially present in lower concentrations, the region of turbidity surrounding the plaques are larger. |
| Morphotype | Siphoviridae |
| Number of Genes | 80 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT771346 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
56 |
| Pitt Freezer Box Grid# |
A7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
