| Detailed Information for Phage Mocha12 |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Alexa Sciarra |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.434509 N, 79.952261 W Map |
| Discovery Notes | The sample was taken on September 11, 2019 at 8:29 P.M. on Parkview Avenue in South Oakland. The sample was taken around lots of grass and weeds. The soil was very grainy and there were many small pebbles. |
| Naming Notes | My phage is named Mocha, which was the name of my old childhood dog. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 24, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2034 |
| Genome length (bp) | 46221 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TACCAGGGGGA |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | This phage is most likely producing lysogens. After reviewing both results from my full plate titers, my 10^-4 dilutions both showed no evidence of phage, meaning that it produced a well grown lawn of Gordonia terrae. However, all of the other dilutions I tested created both a webbed plate and plaques decreasing in dilution as the dilutions got smaller. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | In the uploaded plaque picture, the plaques are about 1-3 mm in size and appear to be lytic because the plaques are very clear. The phage was incubated at 30°C on PYCa plates with host cell Gordonia terrae. |
| Morphotype | Siphoviridae |
| Number of Genes | 75 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW291013 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.33 x 10^10 |
| Pitt Freezer Box# |
114 |
| Pitt Freezer Box Grid# |
G9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
