| Detailed Information for Phage Polly |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Lili Sweeney |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4611 N, 79.954 W Map |
| Discovery Notes | Discovered on 1/26/17 from direct isolation using a soil sample collected from a planter on a 48°F, rainy day. Clonal population for lysate production obtained on 2/14/17. |
| Naming Notes | Nickname of my Great Grandmother, Pauline, which is also my middle name. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 17, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1782 |
| Genome length (bp) | 49780 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques about 1mm in length, occasionally noticeably larger or smaller plaques are present. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK820642 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.3 x 10^10 |
| Date of SEA Lysate Titering |
Mar 2, 2017 |
| Pitt Freezer Box# |
56 |
| Pitt Freezer Box Grid# |
B11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
