| Detailed Information for Phage TimTam |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Krista Roszkowski |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -4°C |
| GPS Coordinates | 40.4465 N, 79.9556 W Map |
| Discovery Notes | Found in a residential area.
Soil was soft, moist, and easy to pick up.
Soil was surrounded by a tree, green plants, and leaves.
The sun was shinning on it. |
| Naming Notes | Tim Tams are my favorite type of cookies from Australia. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 25, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 576 |
| Genome length (bp) | 53431 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Gettysburg College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are about 1.5 mm, cloudy and circular. |
| Morphotype | Siphoviridae |
| Number of Genes | 82 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH479927 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.0 x 10^10 |
| Date of SEA Lysate Titering |
Feb 15, 2016 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
H5 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
