| Detailed Information for Phage Tuti |
| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Maria Castaneda |
| Year Found | 2025 |
| Location Found | Boca Raton, FL United States |
| Finding Institution | Nova Southeastern University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 26.33444 N, 80.14986 W Map |
| Discovery Notes | The soil was collected about 5 feet from the shore of a lake. About 2 inches were dug and then the soil was collected, it was almost completely dry and a bit sandy. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1112 |
| Genome length (bp) | 48255 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCAAGGGGA |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Nova Southeastern University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Plaques are small and clear, this indicates the possibility of a lytic phage. Five plaques were measured; the plaques were 1.136 mm with a standard deviation of 0.16682325976913 |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
5.6 x 10^9 pfu/mL |
| Available Files |
| Plaque Picture | Download |
