| Detailed Information for Phage Twinkle |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Deanna Moore |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -10°C |
| GPS Coordinates | 40.4440088 N, 79.956054 W Map |
| Discovery Notes | Found in a planter outside of Nordenberg Hall. |
| Naming Notes | I let my mom name this phage because she doesn't like anything that has to do with bacteria. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jul 23, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 286 |
| Genome length (bp) | 54241 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCAAGGGGA |
| GC Content | 65.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Has small and large transparent plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK878902 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.0x10^9 |
| Date of SEA Lysate Titering |
Feb 20, 2017 |
| Pitt Freezer Box# |
56 |
| Pitt Freezer Box Grid# |
F7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
