| Discovery Information |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Duplicates of this Phage | DeafIce |
| Found By | Isolated By: Kaitlyn Rogers |
| Year Found | 2020 |
| Location Found | Kirkwood, MO United States |
| Finding Institution | St. Louis Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.567667 N, 90.422083 W Map |
| Discovery Notes | **Phage adopted from Emily Mohn, sample EM602**
Mohn collected the soil sample behind the greenhouse on top of a hill. The GPS coordinates are: 38°34’03.6”N, 90°25’19.5”W. It was very cold outside and there was about 3 inches of snow on the ground. |
| Naming Notes | The name is my dog and significant others names merged together (Winkie and Nick, respectively). It sounds cute, is easy to say, and it has personal meaning to me. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 21, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 348 |
| Genome length (bp) | 47259 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCAAGGGGA |
| GC Content | 66.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | DNA concentration= 119.4 ng/ul
Average Capsid Width= 69.5 nm
Average Tail Length= 336.8 nm
Average Tail Width= 13.5 nm |
| Other Cluster Members |
|
| Annotating Institution | St. Louis Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were small (<1-2 mm) and rounded. Titer performed to 10^-8. Chose from titer of 10^-7, only two plagues overlapped, the rest were spread out; total of 6 plaques counted. Obtained that there are 2x10^10 phage per mL. |
| Morphotype | Siphoviridae |
| Number of Genes | 80 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON260830 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
10^-8 |
| Pitt Freezer Box# |
127 |
| Pitt Freezer Box Grid# |
C8 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
