| Detailed Information for Phage Antonio |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Hannah Madara |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4432 N, 79.9597 W Map |
| Discovery Notes | This phage was found from a soil sample collected on the corner of O'Hara street and De Soto Street, outside of a UPMC building in what appeared to be a flower bed. The weather was cold, 20 degrees F, and there was snow on the ground. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 12, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1930 |
| Genome length (bp) | 50337 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques vary in size from .5mm up to 3mm. They are all completely clear and appear circular in size. |
| Morphotype | Siphoviridae |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK814755 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.67 x 10^10 pfu/mL |
| Date of SEA Lysate Titering |
Feb 14, 2017 |
| Pitt Freezer Box# |
55 |
| Pitt Freezer Box Grid# |
C6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
