| Detailed Information for Phage Archie |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rose M. Clarke |
| Year Found | 2009 |
| Location Found | Corvallis, OR USA |
| Finding Institution | Oregon State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.567722 N, 123.281986 W Map |
| Discovery Notes | Moist soil, depth 3 cm, in downspout runoff gully filled with dense ground cover and low-growing flowers. West Cordley Garden. 60°F |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 25, 2011 |
| Genome length (bp) | 76271 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 58.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are small, about 1-2mm in diameter; all have defined “bulls eye” pattern, with center completely clear surrounded by ring of cloudy clearing; plates incubated at 37° for at least 24 hours |
| Morphotype | Siphoviridae |
| Number of Genes | 128 |
| Number of tRNAs | 12 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT591489 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2009ORSUArchieRMC |
| SEA Lysate Titer |
2.5e10 |
| Date of SEA Lysate Titering |
Nov 17, 2009 |
| Pitt Freezer Box# |
5 |
| Pitt Freezer Box Grid# |
39 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
