Mycobacterium phage Crossroads
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Detailed Information for Phage Crossroads
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesCrossroads2K
Found ByWayne Sera
Year Found2011
Location FoundNorth Huntingdon, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.326475 N, 79.677855 W Map
Discovery Notes*** Crossroads2K became the 2000th phage added to the database on 11.29.11! ***
Naming Notes"Crossroads" is from a classic Robert Johnson song. It is the 2,000th phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 25, 2012
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage131
Sanger Finishing Reactions14
Genome length (bp)76129
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesOriginal enriched sample yield 1 plaque, approximately 1 mm in size.
MorphotypeSiphoviridae
Number of Genes133
Number of tRNAs12
Has been Phamerated?Yes
Gene List

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Crossroads_1

Crossroads_2

Crossroads_3

Crossroads_4
(terminase)

Crossroads_5
(portal protein)

Crossroads_6

Crossroads_7
(scaffolding protein)

Crossroads_8
(Capsid)

Crossroads_9

Crossroads_10

Crossroads_11

Crossroads_12

Crossroads_13
(major tail subunit)

Crossroads_14
(tail assembly chaperone)

Crossroads_15
(tail assembly chaperone)

Crossroads_16
(tape measure protein)

Crossroads_17
(minor tail subunit)

Crossroads_18
(minor tail subunit)

Crossroads_19

Crossroads_20

Crossroads_21

Crossroads_22

Crossroads_23

Crossroads_24

Crossroads_25

Crossroads_26

Crossroads_27
(LysA)

Crossroads_28
(LysB)

Crossroads_29

Crossroads_30

Crossroads_31

Crossroads_32
(NrdH-like protein)

Crossroads_33

Crossroads_34

Crossroads_35
(NrdC-like protein)

Crossroads_36
(Lsr2)

Crossroads_37

Crossroads_38
(integrase)

Crossroads_39

Crossroads_40

Crossroads_41

Crossroads_42

Crossroads_43

Crossroads_44

Crossroads_45

Crossroads_46

Crossroads_47

Crossroads_48

Crossroads_49

Crossroads_50

Crossroads_51

Crossroads_52

Crossroads_53

Crossroads_54

Crossroads_55
(Von Willebrand-like domain protein)

Crossroads_56

Crossroads_57

Crossroads_58

Crossroads_59

Crossroads_60

Crossroads_61

Crossroads_62

Crossroads_63

Crossroads_64

Crossroads_65

Crossroads_66

Crossroads_67

Crossroads_68

Crossroads_69

Crossroads_70
(DnaC helicase)

Crossroads_71

Crossroads_72

Crossroads_73

Crossroads_74
(phosphoribosyl-pyrophosphate synthetase)

Crossroads_75
(NAPRTase)

Crossroads_76
(PNK protein)

Crossroads_77

Crossroads_78

Crossroads_79

Crossroads_80
(RusA)

Crossroads_81
(WhiB)

Crossroads_82
(WhiB)

Crossroads_83

Crossroads_84

Crossroads_85
(ssDNA binding protein)

Crossroads_86

Crossroads_87

Crossroads_88
(RNA ligase)

Crossroads_89

Crossroads_90
(ClpP)

Crossroads_91

Crossroads_92

Crossroads_93

Crossroads_94

Crossroads_95
(HNH endonuclease)

Crossroads_96

Crossroads_97

Crossroads_98
(DnaQ-like protein)

Crossroads_99

Crossroads_100

Crossroads_101

Crossroads_102

Crossroads_103

Crossroads_104

Crossroads_105

Crossroads_106

Crossroads_107

Crossroads_109

Crossroads_110

Crossroads_113

Crossroads_120

Crossroads_121

Crossroads_123

Crossroads_125

Crossroads_127

Crossroads_128

Crossroads_129

Crossroads_130

Crossroads_131

Crossroads_132

Crossroads_133

Crossroads_134

Crossroads_135

Crossroads_136

Crossroads_137

Crossroads_138

Crossroads_139

Crossroads_140

Crossroads_141
(AAA ATPase)

Crossroads_142

Crossroads_143

Crossroads_144

Crossroads_145

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF024731
Refseq NumberNC_022071
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
Pitt Freezer Box# 1
Pitt Freezer Box Grid# E8
Available Files
EM PictureDownload
Final DNAMaster FileDownload