Mycobacterium phage Halena
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Detailed Information for Phage Halena
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByHaley Hammel and Alyna Fernandes
Year Found2017
Location FoundNorth Andover, MA United States
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates42.6705 N, 71.1236 W Map
Discovery NotesOur sample was collected in local moist soil on the Merrimack Campus.
Naming NotesThe name is a combination of both of our names, "Ha" from Haley and "lena" from Alyna.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 19, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage701
Genome length (bp)73871
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesAll of our phage were circular and about the same size. They were all clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Halena_1

Halena_2
(terminase, small subunit)

Halena_3

Halena_4
(terminase, large subunit)

Halena_5
(portal protein)

Halena_6
(MuF-like minor capsid protein)

Halena_7
(scaffolding protein)

Halena_8
(major capsid protein)

Halena_9
(head-to-tail adaptor)

Halena_10
(head-to-tail stopper)

Halena_11

Halena_12
(tail terminator)

Halena_13
(major tail protein)

Halena_14
(tail assembly chaperone)

Halena_15
(tail assembly chaperone)

Halena_16
(tape measure protein)

Halena_17
(minor tail protein)

Halena_18
(minor tail protein)

Halena_19
(minor tail protein)

Halena_20
(minor tail protein)

Halena_21
(minor tail protein)

Halena_22

Halena_23

Halena_24

Halena_25
(lysin A)

Halena_26
(lysin B)

Halena_27
(holin)

Halena_28
(membrane protein)

Halena_29
(membrane protein)

Halena_30
(NrdH-like glutaredoxin)

Halena_31
(membrane protein)

Halena_32
(Nucleotidyl transferase)

Halena_33
(Lsr2-like DNA bridging protein)

Halena_34

Halena_35
(integrase, (Y-int))

Halena_36
(membrane protein)

Halena_37

Halena_38
(DNA binding protein)

Halena_39
(excise)

Halena_40

Halena_41
(membrane protein)

Halena_42

Halena_43

Halena_44

Halena_45
(esterase)

Halena_46

Halena_47

Halena_48

Halena_49

Halena_50

Halena_51

Halena_52

Halena_53

Halena_54
(membrane protein)

Halena_55

Halena_56

Halena_57
(Cas4-family exonuclease)

Halena_58

Halena_59
(ssDNA binding protein, ERF family)

Halena_60

Halena_61

Halena_62

Halena_63
(membrane protein)

Halena_64
(HNH endonuclease)

Halena_65

Halena_66
(DnaB-like dsDNA helicase)

Halena_67

Halena_68

Halena_69

Halena_70
(phosphoribosyl transferase)

Halena_71
(nicotinate ribosyltransferase)

Halena_72

Halena_73
(polynucleotide kinase)

Halena_74

Halena_75
(RusA-like resolvase (endonuclease))

Halena_76
(WhiB family transcription factor)

Halena_77
(WhiB family transcription factor)

Halena_78

Halena_79

Halena_80
(ssDNA binding protein)

Halena_81

Halena_82

Halena_83
(RNA ligase)

Halena_84

Halena_85

Halena_86

Halena_87

Halena_88
(HNH endonuclease)

Halena_89

Halena_90

Halena_91
(DnaQ-like exonuclease (DNA polymerase III subunit))

Halena_92

Halena_93

Halena_94

Halena_95
(endonuclease VII)

Halena_96
(HNH endonuclease)

Halena_97

Halena_98

Halena_99

Halena_100

Halena_101
(membrane protein)

Halena_104

Halena_108

Halena_109

Halena_111

Halena_112

Halena_114

Halena_116

Halena_117

Halena_118

Halena_119

Halena_120

Halena_121

Halena_122

Halena_123

Halena_124

Halena_125

Halena_126

Halena_127

Halena_128

Halena_129
(AAA-ATPase)

Halena_130

Halena_131

Halena_132

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK494100
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 73
Pitt Freezer Box Grid# H9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload