Mycobacterium phage Archie
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Detailed Information for Phage Archie
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRose M. Clarke
Year Found2009
Location FoundCorvallis, OR USA
Finding InstitutionOregon State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.567722 N, 123.281986 W Map
Discovery NotesMoist soil, depth 3 cm, in downspout runoff gully filled with dense ground cover and low-growing flowers. West Cordley Garden. 60°F
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 25, 2011
Genome length (bp)76271
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesPlaques are small, about 1-2mm in diameter; all have defined “bulls eye” pattern, with center completely clear surrounded by ring of cloudy clearing; plates incubated at 37° for at least 24 hours
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Archie_1

Archie_2
(terminase small subunit)

Archie_3

Archie_4
(terminase large subunit)

Archie_5
(portal protein)

Archie_6

Archie_7
(scaffolding protein)

Archie_8
(major capsid protein)

Archie_9

Archie_10

Archie_11

Archie_12

Archie_13
(major tail protein)

Archie_14
(tail assembly chaperone)

Archie_15
(tail assembly chaperone)

Archie_16
(tape measure protein)

Archie_17
(minor tail protein)

Archie_18
(minor tail protein)

Archie_19
(minor tail protein)

Archie_20
(minor tail protein)

Archie_21

Archie_22

Archie_23

Archie_24
(minor tail protein)

Archie_25

Archie_26

Archie_27
(lysin A)

Archie_28
(lysin B)

Archie_29

Archie_30

Archie_31

Archie_32
(glutaredoxin)

Archie_33

Archie_34

Archie_35
(nucleotidyl transferase)

Archie_36

Archie_37

Archie_38
(integrase)

Archie_39

Archie_40
(immunity repressor)

Archie_41
(helix-turn-helix DNA binding domain protein)

Archie_42
(excise)

Archie_43

Archie_44

Archie_45

Archie_46

Archie_47
(esterase)

Archie_48

Archie_49

Archie_50

Archie_51

Archie_52

Archie_53

Archie_54

Archie_55

Archie_56

Archie_57

Archie_58

Archie_59

Archie_60

Archie_61

Archie_62

Archie_63

Archie_64

Archie_65

Archie_66

Archie_67
(HNH endonuclease)

Archie_68

Archie_69
(dsDNA helicase)

Archie_70

Archie_71

Archie_72
(phosphoribosyl pyrophosphate synthetase)

Archie_73
(nicotinate ribosyltransferase)

Archie_74
(polynucleotide kinase)

Archie_75

Archie_76

Archie_77
(resolvase)

Archie_78
(WhiB family transcription factor)

Archie_79
(WhiB family transcription factor)

Archie_80

Archie_81

Archie_82
(ssDNA binding protein)

Archie_83

Archie_84

Archie_85
(RNA ligase)

Archie_86

Archie_87
(ClpP protease)

Archie_88

Archie_89

Archie_90

Archie_91

Archie_92
(HNH endonuclease)

Archie_93

Archie_94

Archie_95
(DNA polymerase III subunit)

Archie_96

Archie_97

Archie_98
(HNH endonuclease)

Archie_99

Archie_100

Archie_101

Archie_102

Archie_104

Archie_107

Archie_108

Archie_115

Archie_116

Archie_118

Archie_119

Archie_121

Archie_123

Archie_124

Archie_125

Archie_126

Archie_127

Archie_128

Archie_129

Archie_130

Archie_131

Archie_132

Archie_133

Archie_134

Archie_135

Archie_136

Archie_137

Archie_138
(AAA-ATPase)

Archie_139

Archie_140

Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT591489
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Designator 2009ORSUArchieRMC
SEA Lysate Titer 2.5e10
Date of SEA Lysate Titering Nov 17, 2009
Pitt Freezer Box# 5
Pitt Freezer Box Grid# 39
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload