Mycobacterium phage OhShagHennessy
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Detailed Information for Phage OhShagHennessy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCaitlyn Cason
Year Found2019
Location FoundBlacksburg, VA United States
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.2249 N, 80.4183 W Map
Discovery NotesFound outside of O'Shaughnessy Hall, a dorm on Virginia Tech's campus. It was in an area well maintained but not routinely walked on by students. The area was not in close proximity to animals, large trees or bushes, or bodies of water.
Naming NotesAs it was found outside of O'Shaughnessy Hall I named my phage after a play on words of the dorm name. There is a popular Key and Peele skit called Substitute Teacher where the consistently mispronounce names, including O'Shaughnessy.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1366
Genome length (bp)72623
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Tech
Annotation StatusIn GenBank
Plaque NotesThe plaques were small in size but incredibly clear. There were no halos on any plates or turbidity that was visible. The clear plaques with defined edges made harvesting them easier.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

OhShagHennessy_1

OhShagHennessy_2
(terminase small subunit)

OhShagHennessy_3

OhShagHennessy_4
(terminase large subunit)

OhShagHennessy_5
(portal protein)

OhShagHennessy_6
(MuF-like minor capsid protein)

OhShagHennessy_7
(scaffolding protein)

OhShagHennessy_8
(major capsid protein)

OhShagHennessy_9
(head-to-tail adaptor)

OhShagHennessy_10
(head-to-tail stopper)

OhShagHennessy_11

OhShagHennessy_12
(tail terminator)

OhShagHennessy_13
(major tail protein)

OhShagHennessy_14
(tail assembly chaperone)

OhShagHennessy_15
(tail assembly chaperone)

OhShagHennessy_16
(tape measure protein)

OhShagHennessy_17
(minor tail protein)

OhShagHennessy_18
(minor tail protein)

OhShagHennessy_19
(minor tail protein)

OhShagHennessy_20
(minor tail protein)

OhShagHennessy_21
(minor tail protein)

OhShagHennessy_22

OhShagHennessy_23

OhShagHennessy_24

OhShagHennessy_25
(lysin A)

OhShagHennessy_26
(lysin B)

OhShagHennessy_27

OhShagHennessy_28

OhShagHennessy_29

OhShagHennessy_30
(NrdH-like glutaredoxin)

OhShagHennessy_31

OhShagHennessy_32
(nucleotidyltransferase)

OhShagHennessy_33

OhShagHennessy_34

OhShagHennessy_35
(tyrosine integrase)

OhShagHennessy_36

OhShagHennessy_37
(immunity repressor)

OhShagHennessy_38
(Cro protein)

OhShagHennessy_39
(excise)

OhShagHennessy_40

OhShagHennessy_41

OhShagHennessy_42

OhShagHennessy_43

OhShagHennessy_44

OhShagHennessy_45
(esterase)

OhShagHennessy_46

OhShagHennessy_47

OhShagHennessy_48

OhShagHennessy_49

OhShagHennessy_50

OhShagHennessy_51

OhShagHennessy_52

OhShagHennessy_53

OhShagHennessy_54

OhShagHennessy_55

OhShagHennessy_56

OhShagHennessy_57
(Cas4 family exonuclease)

OhShagHennessy_58

OhShagHennessy_59
(ERF family ssDNA binding protein)

OhShagHennessy_60

OhShagHennessy_61

OhShagHennessy_62

OhShagHennessy_63

OhShagHennessy_64

OhShagHennessy_65

OhShagHennessy_66
(DnaB-like dsDNA helicase)

OhShagHennessy_67

OhShagHennessy_68

OhShagHennessy_69

OhShagHennessy_70
(phosphoribosyl pyrophosphate transferase)

OhShagHennessy_71
(nicotinate ribosyltransferase)

OhShagHennessy_72

OhShagHennessy_73
(polynucleotide kinase)

OhShagHennessy_74

OhShagHennessy_75

OhShagHennessy_76
(WhiB family transcription factor)

OhShagHennessy_77

OhShagHennessy_78

OhShagHennessy_79
(ssDNA binding protein)

OhShagHennessy_80

OhShagHennessy_81

OhShagHennessy_82
(RNA ligase)

OhShagHennessy_83

OhShagHennessy_84

OhShagHennessy_85

OhShagHennessy_86

OhShagHennessy_87
(HNH endonuclease)

OhShagHennessy_88

OhShagHennessy_89

OhShagHennessy_90
(DnaQ-like DNA polymerase III subunit)

OhShagHennessy_91

OhShagHennessy_92

OhShagHennessy_93
(HNH endonuclease)

OhShagHennessy_94

OhShagHennessy_95

OhShagHennessy_96

OhShagHennessy_97

OhShagHennessy_100

OhShagHennessy_105

OhShagHennessy_106

OhShagHennessy_108

OhShagHennessy_109

OhShagHennessy_110

OhShagHennessy_111

OhShagHennessy_112

OhShagHennessy_113

OhShagHennessy_114

OhShagHennessy_115

OhShagHennessy_116

OhShagHennessy_117

OhShagHennessy_118

OhShagHennessy_119

OhShagHennessy_120

OhShagHennessy_121
(AAA-ATPase)

OhShagHennessy_122

OhShagHennessy_123

OhShagHennessy_124

OhShagHennessy_125

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW435855
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 102
Pitt Freezer Box Grid# B9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload