Mycobacterium phage OhShagHennessy
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Detailed Information for Phage OhShagHennessy
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCaitlyn Cason
Year Found2019
Location FoundBlacksburg, VA United States
Finding InstitutionVirginia Tech
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates37.2249 N, 80.4183 W Map
Discovery NotesFound outside of O'Shaughnessy Hall, a dorm on Virginia Tech's campus. It was in an area well maintained but not routinely walked on by students. The area was not in close proximity to animals, large trees or bushes, or bodies of water.
Naming NotesAs it was found outside of O'Shaughnessy Hall I named my phage after a play on words of the dorm name. There is a popular Key and Peele skit called Substitute Teacher where the consistently mispronounce names, including O'Shaughnessy.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1366
Genome length (bp)72623
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionVirginia Tech
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesThe plaques were small in size but incredibly clear. There were no halos on any plates or turbidity that was visible. The clear plaques with defined edges made harvesting them easier.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload