Mycobacterium phage Lolly9

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Detailed Information for Phage Lolly9
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLondiwe Ntshalintshali
Year Found2013
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Discovery Notesgrassy area of parking lot with compost.after two days of enrichment,first round purification and the titre is 4x1014 pfu/ml
Naming Notesafter my nickname lolly and 9 is for my birth day
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 8, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage525
Genome length (bp)75816
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.3%
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterL
SubclusterL3
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque Notessmall clear and circular plaques
MorphotypeSiphoviridae
Number of Genes130
Number of tRNAs11
Has been Phamerated?Yes
Gene List

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Lolly9_1

Lolly9_2

Lolly9_3

Lolly9_4

Lolly9_5
(Terminase large subunit)

Lolly9_6
(Portal)

Lolly9_7

Lolly9_8
(Scaffolding protein)

Lolly9_9
(Major Capsid)

Lolly9_10

Lolly9_11

Lolly9_12

Lolly9_13

Lolly9_14
(Major Tail)

Lolly9_15
(Tail assembly chaperone)

Lolly9_16
(Tail assembly chaperone)

Lolly9_17
(Tape measure)

Lolly9_18
(Minor tail protein)

Lolly9_19
(Minor tail protein)

Lolly9_20
(Minor tail protein)

Lolly9_21
(Minor tail protein)

Lolly9_22
(Minor tail protein)

Lolly9_23

Lolly9_24

Lolly9_25

Lolly9_26
(Lysin A)

Lolly9_27

Lolly9_28
(Lysin B)

Lolly9_29

Lolly9_30

Lolly9_31
(NrdH glutaredoxin protein)

Lolly9_32

Lolly9_33
(Nucleotidyltransferase)

Lolly9_34
(Lsr2 DNA binding protein)

Lolly9_35

Lolly9_36
(Integrase (Y-int))

Lolly9_37

Lolly9_38
(Immunity repressor)

Lolly9_39

Lolly9_40
(DNA binding protein)

Lolly9_41

Lolly9_42

Lolly9_43

Lolly9_44

Lolly9_45

Lolly9_46

Lolly9_47

Lolly9_48

Lolly9_50

Lolly9_51

Lolly9_52

Lolly9_53

Lolly9_54
(RNA binding protein)

Lolly9_55

Lolly9_56

Lolly9_57

Lolly9_58

Lolly9_59

Lolly9_60

Lolly9_61

Lolly9_62
(ssDNA bindiing protein ERF family)

Lolly9_63

Lolly9_64

Lolly9_65
(HNH endonuclease)

Lolly9_66

Lolly9_67

Lolly9_68

Lolly9_69
(DNA helicase)

Lolly9_70

Lolly9_71

Lolly9_72

Lolly9_73
(ribose-phosphate pyrophosphokinase)

Lolly9_74
(Nicotinamide ribosyltransferase)

Lolly9_75

Lolly9_76
(Polynucleotide kinase)

Lolly9_77
(HJ Resolvase RusA-like protein)

Lolly9_78
(WhiB family transcription factor)

Lolly9_79
(WhiB family transcription factor)

Lolly9_80

Lolly9_81

Lolly9_82
(ssDNA binding protein)

Lolly9_83

Lolly9_84

Lolly9_85
(RNA ligase)

Lolly9_86
(CliP protease)

Lolly9_87

Lolly9_88

Lolly9_89

Lolly9_90

Lolly9_91
(HNH endonuclease)

Lolly9_92

Lolly9_93

Lolly9_94
(DNA-Q exonuclease)

Lolly9_95

Lolly9_96

Lolly9_97

Lolly9_98

Lolly9_99

Lolly9_100

Lolly9_101

Lolly9_103

Lolly9_105

Lolly9_110

Lolly9_113

Lolly9_114

Lolly9_115

Lolly9_118

Lolly9_119

Lolly9_120

Lolly9_121

Lolly9_122

Lolly9_123

Lolly9_124

Lolly9_125

Lolly9_126

Lolly9_127

Lolly9_128

Lolly9_129

Lolly9_130

Lolly9_131

Lolly9_132

Lolly9_133

Lolly9_134
(AAA ATPase)

Lolly9_135

Lolly9_136

Lolly9_137

Lolly9_138

Lolly9_139

Lolly9_140

Lolly9_141

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT281791
Refseq NumberNC_028843
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload