Mycobacterium phage Lumos
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Detailed Information for Phage Lumos
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLaura Molina
Year Found2014
Location FoundStuart, FL USA
Finding InstitutionIndian River State College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature29°C
GPS Coordinates27.176074 N, 80.252566 W Map
Discovery NotesSoil collected from Moore's Cemetery in Stuart, FL. The soil was dry and mixed heavily with sand.
Naming NotesLumos refers to the spell for light in "Harry Potter"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 10, 2014
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage185
Genome length (bp)75586
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL3
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesClear, all around, approximately 3-4mm in diameter
MorphotypeSiphoviridae
Number of Genes128
Number of tRNAs10
Has been Phamerated?Yes
Gene List

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Lumos_1

Lumos_2
(terminase small subunit)

Lumos_3

Lumos_4

Lumos_5
(terminase large subunit)

Lumos_6
(portal protein)

Lumos_7
(capsid maturation protease)

Lumos_8
(scaffolding protein)

Lumos_9
(capsid protein)

Lumos_10
(head-tail connector)

Lumos_11
(head-tail connector)

Lumos_12
(head-tail connector)

Lumos_13

Lumos_14
(major tail protein)

Lumos_15
(tail assembly chaperone)

Lumos_16
(tail assembly chaperone)

Lumos_17
(tapemeasure)

Lumos_18
(minor tail protein)

Lumos_19
(minor tail protein)

Lumos_20
(minor tail protein)

Lumos_21

Lumos_22

Lumos_23

Lumos_24

Lumos_25

Lumos_26
(lysin A)

Lumos_27

Lumos_28
(lysin B)

Lumos_29

Lumos_30

Lumos_31
(glutaredoxin NrdH-like protein)

Lumos_32

Lumos_33
(nucleotidyltransferase-like protein)

Lumos_34
(Lsr2)

Lumos_35

Lumos_36
(integrase)

Lumos_37

Lumos_38

Lumos_39
(DNA binding protein)

Lumos_40

Lumos_41

Lumos_42

Lumos_43

Lumos_44

Lumos_45
(metallophosphoesterase)

Lumos_46

Lumos_47

Lumos_48

Lumos_49
(band 7-like protein)

Lumos_51

Lumos_52

Lumos_53

Lumos_54

Lumos_55

Lumos_56

Lumos_57

Lumos_58

Lumos_59

Lumos_60

Lumos_61

Lumos_62

Lumos_63
(ERF-like DNA single-strand annealing protein)

Lumos_64

Lumos_65
(DNA binding domain protein)

Lumos_66

Lumos_67
(HNH endonuclease)

Lumos_68

Lumos_69

Lumos_70
(DnaB-like replicative helicase)

Lumos_71
(WhiB)

Lumos_72

Lumos_73

Lumos_74

Lumos_75
(phosphoribosyl pyrophosphate synthetase)

Lumos_76
(phosphoribosyl transferase)

Lumos_77
(polynucleotide kinase)

Lumos_78

Lumos_79
(endodeoxyribonuclease RusA)

Lumos_80
(WhiB)

Lumos_81

Lumos_82

Lumos_83

Lumos_84
(ssDNA binding protein)

Lumos_85

Lumos_86
(RNA ligase)

Lumos_87
(CLP protease)

Lumos_88

Lumos_89

Lumos_90

Lumos_91
(DNA binding protein)

Lumos_92
(HNH endonuclease)

Lumos_93

Lumos_94

Lumos_95
(DNA polymerase)

Lumos_96

Lumos_97

Lumos_98
(HNH endonuclease)

Lumos_99

Lumos_100

Lumos_101

Lumos_102

Lumos_104

Lumos_106

Lumos_110

Lumos_113

Lumos_114

Lumos_117

Lumos_118

Lumos_119

Lumos_120

Lumos_121

Lumos_122

Lumos_123

Lumos_124

Lumos_125

Lumos_126

Lumos_127

Lumos_128

Lumos_129

Lumos_130

Lumos_131

Lumos_132

Lumos_133

Lumos_134
(AAA ATPase)

Lumos_135

Lumos_136

Lumos_137

Lumos_138

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT372003
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.99x10^9
Date of SEA Lysate Titering Oct 22, 2014
Pitt Freezer Box# 10
Pitt Freezer Box Grid# G11