Mycobacterium phage Acquire49
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Detailed Information for Phage Acquire49
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTyler Burton
Year Found2016
Location FoundNancy, KY USA
Finding InstitutionSomerset Community College
ProgramKentucky Biomedical Infrastructure Network Small Genomes Discovery Program
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.082778 N, 84.101389 W Map
Discovery NotesTemperature: around 30 degrees F or below.
Date: February 15, 2016
Climate: It was snowy, damp, and cold.
Characteristics of soil: It was damp and the color of the soil was dark, but the soil had a soft texture to it.

From: Same enrichment as PhantomSKS
Naming NotesTo be honest I sat down with my girlfriend and told her to name off random letters and numbers with the first character being capital. She came up with A3cq49. For the Database it was named Acquire49.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 23, 2016
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage407
Genome length (bp)73639
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionSomerset Community College
Annotation StatusIn GenBank
Plaque NotesClear, very round, lytic in nature. Approximately 2mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Acquire49_1

Acquire49_2
(terminase small subunit)

Acquire49_3

Acquire49_4
(terminase large subunit)

Acquire49_5
(portal protein)

Acquire49_6
(capsid maturation protease)

Acquire49_7
(scaffolding protein)

Acquire49_8
(major capsid protein)

Acquire49_9
(head-to-tail adaptor)

Acquire49_10
(head-to-tail stopper)

Acquire49_11

Acquire49_12
(tail terminator)

Acquire49_13
(major tail protein)

Acquire49_14
(tail assembly chaperone)

Acquire49_15
(tail assembly chaperone)

Acquire49_16
(tape measure protein)

Acquire49_17
(minor tail protein)

Acquire49_18
(minor tail protein)

Acquire49_19
(minor tail protein)

Acquire49_20
(minor tail protein)

Acquire49_21
(minor tail protein)

Acquire49_22

Acquire49_23

Acquire49_24

Acquire49_25
(lysin A)

Acquire49_26
(lysin B)

Acquire49_27

Acquire49_28
(holin)

Acquire49_29
(membrane protein)

Acquire49_30
(NrdH-like glutaredoxin)

Acquire49_31

Acquire49_32
(nucleotidyl transferase)

Acquire49_33
(Lsr2-like DNA bridging protein)

Acquire49_34

Acquire49_35

Acquire49_36
(integrase)

Acquire49_37
(membrane protein)

Acquire49_38
(immunity repressor)

Acquire49_39
(Cro protein)

Acquire49_40
(excise)

Acquire49_41

Acquire49_42

Acquire49_43

Acquire49_44

Acquire49_45

Acquire49_46
(esterase)

Acquire49_47

Acquire49_48

Acquire49_49
(band-7 -like membrane protein)

Acquire49_50

Acquire49_51

Acquire49_52

Acquire49_53

Acquire49_54

Acquire49_55

Acquire49_56

Acquire49_57

Acquire49_58

Acquire49_59
(exonuclease)

Acquire49_60
(antirepressor)

Acquire49_61
(ERF family ssDNA binding protein)

Acquire49_62

Acquire49_63

Acquire49_64

Acquire49_65
(membrane protein)

Acquire49_66

Acquire49_67

Acquire49_68
(DnaB-like dsDNA helicase)

Acquire49_69
(WhiB family transcription factor)

Acquire49_70

Acquire49_71

Acquire49_72
(phosphoribosyl transferase)

Acquire49_73
(nicotinate ribosyltransferase)

Acquire49_74

Acquire49_75
(polynucleotide kinase)

Acquire49_76

Acquire49_77
(RusA-like resolvase)

Acquire49_78
(WhiB family transcription factor)

Acquire49_79
(WhiB family transcription factor)

Acquire49_80

Acquire49_81

Acquire49_82
(ssDNA binding protein)

Acquire49_83

Acquire49_84
(membrane protein)

Acquire49_85

Acquire49_86
(RNA ligase)

Acquire49_87

Acquire49_88

Acquire49_89

Acquire49_90

Acquire49_91
(HNH endonuclease)

Acquire49_92

Acquire49_93

Acquire49_94
(DnaQ-like exonuclease)

Acquire49_95

Acquire49_96

Acquire49_97
(endonuclease VII)

Acquire49_98
(HNH endonuclease)

Acquire49_99

Acquire49_100

Acquire49_101

Acquire49_102

Acquire49_105

Acquire49_111

Acquire49_112

Acquire49_114

Acquire49_116

Acquire49_117

Acquire49_118

Acquire49_119

Acquire49_120

Acquire49_121

Acquire49_122

Acquire49_123

Acquire49_124

Acquire49_125

Acquire49_126

Acquire49_127

Acquire49_128

Acquire49_129
(AAA-ATPase)

Acquire49_130

Acquire49_131

Acquire49_132

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK279905
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.8x10^10 pfu/mL
Date of SEA Lysate Titering Apr 26, 2016
Pitt Freezer Box# 28
Pitt Freezer Box Grid# A10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload