Mycobacterium phage Zakai
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Detailed Information for Phage Zakai
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByZakai Meghoo-Peddie
Year Found2015
Location FoundAtlanta, GA United States
Finding InstitutionMorehouse College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.748116 N, 84.414223 W Map
Discovery Notescollected from a rocky and dry type of soil on a very warm and dry day. Using cluster specific PCR primers indicated that this is a Cluster A phage.
Naming NotesThe name of the phage comes from the name Zakai which means pure and innocent.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1989
Genome length (bp)76363
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesClear and round plaques.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Zakai_1

Zakai_2
(terminase small subunit)

Zakai_3

Zakai_4
(terminase large subunit)

Zakai_5
(portal protein)

Zakai_6
(capsid maturation protease)

Zakai_7
(scaffolding protein)

Zakai_8
(major capsid protein)

Zakai_9
(head-to-tail connector protein)

Zakai_10
(head-to-tail connector protein)

Zakai_11
(head-to-tail connector protein)

Zakai_12
(head-to-tail connector protein)

Zakai_13
(major tail protein)

Zakai_14
(tail assembly chaperone)

Zakai_15
(tail assembly chaperone)

Zakai_16
(tape measure protein)

Zakai_17
(minor tail protein)

Zakai_18
(minor tail protein)

Zakai_19
(minor tail protein)

Zakai_20
(minor tail protein)

Zakai_21
(minor tail protein)

Zakai_22
(minor tail protein)

Zakai_23
(minor tail protein)

Zakai_24
(minor tail protein)

Zakai_25

Zakai_26

Zakai_27
(lysin A)

Zakai_28
(lysin B)

Zakai_29

Zakai_30

Zakai_31

Zakai_32
(glutaredoxin)

Zakai_33

Zakai_34

Zakai_35
(nucleotidyltransferase)

Zakai_36
(DNA bridging protein)

Zakai_37

Zakai_38
(integrase)

Zakai_39

Zakai_40
(immunity repressor)

Zakai_41
(CRO protein)

Zakai_42
(excise)

Zakai_43

Zakai_44

Zakai_45

Zakai_46

Zakai_47
(esterase)

Zakai_48

Zakai_49

Zakai_50

Zakai_51

Zakai_52
(band-7-like membrane protein)

Zakai_53

Zakai_54

Zakai_55

Zakai_57

Zakai_58

Zakai_59

Zakai_60

Zakai_61

Zakai_62

Zakai_63

Zakai_64
(ERF family ssDNA binding protein)

Zakai_65

Zakai_66

Zakai_67

Zakai_68

Zakai_69

Zakai_70

Zakai_71
(dsDNA helicase)

Zakai_72

Zakai_73

Zakai_74

Zakai_75
(phosphoribosyl pyrophosphate transferase)

Zakai_76
(nicotinate phosphoribosyltransferase)

Zakai_77
(polynucleotide kinase)

Zakai_78

Zakai_79

Zakai_80

Zakai_81
(endonuclease)

Zakai_82
(WhiB family transcription factor)

Zakai_83
(WhiB family transcription factor)

Zakai_84

Zakai_85
(HTH DNA binding protein)

Zakai_86
(ssDNA binding protein)

Zakai_87

Zakai_88

Zakai_89
(RNA ligase)

Zakai_90

Zakai_91
(protease)

Zakai_92

Zakai_93

Zakai_94

Zakai_95

Zakai_96
(HNH endonuclease)

Zakai_97

Zakai_98

Zakai_99
(DNA polymerase III subunit)

Zakai_100

Zakai_101

Zakai_102
(HNH endonuclease)

Zakai_103

Zakai_104

Zakai_105

Zakai_107

Zakai_111

Zakai_112

Zakai_119

Zakai_120

Zakai_122

Zakai_123

Zakai_125

Zakai_127

Zakai_128

Zakai_129

Zakai_130

Zakai_131

Zakai_132

Zakai_133

Zakai_134

Zakai_135

Zakai_136

Zakai_137

Zakai_138

Zakai_139

Zakai_140

Zakai_141
(AAA-ATPase)

Zakai_142

Zakai_143

Zakai_144

Zakai_145

Zakai_146

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX580961
Refseq NumberNone yet
Published in PaperHerren et al. 2017, Genome Announcements: Genome Sequences of Four Subcluster L2 Mycobacterium Phages, Finemlucis, Miley16, Wilder, and Zakai
Archiving Info
Archiving status Archived
Pitt Freezer Box# 21
Pitt Freezer Box Grid# C4
Available Files
Final DNAMaster FileDownload
GenBank File for PhameratorDownload