Mycobacterium phage Silverleaf
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Detailed Information for Phage Silverleaf
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByRaistland Valenzuela and Hari Kotturi
Year Found2017
Location FoundOklahoma City, OK USA
Finding InstitutionUniversity of Central Oklahoma
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates35.584167 N, 97.474167 W Map
Discovery NotesThe soil was collected underneath pine trees. The soil was moist.
Naming NotesAddress
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 1, 2017
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage371
Genome length (bp)73210
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Central Oklahoma
Annotation StatusIn GenBank
Plaque NotesThe phage forms plaques clear plaques with 3mm diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Silverleaf_1

Silverleaf_2
(terminase small subunit)

Silverleaf_3

Silverleaf_4
(terminase large subunit)

Silverleaf_5
(portal protein)

Silverleaf_6
(capsid maturation protease)

Silverleaf_7
(scaffolding protein)

Silverleaf_8
(major capsid protein)

Silverleaf_9
(head-to-tail adaptor)

Silverleaf_10
(head-to-tail stopper)

Silverleaf_11

Silverleaf_12
(tail terminator)

Silverleaf_13
(major tail protein)

Silverleaf_14
(tail assembly chaperone)

Silverleaf_15
(tail assembly chaperone)

Silverleaf_16
(tapemeasure protein)

Silverleaf_17
(minor tail protein)

Silverleaf_18
(minor tail protein)

Silverleaf_19
(minor tail protein)

Silverleaf_20
(minor tail protein)

Silverleaf_21
(minor tail protein)

Silverleaf_22

Silverleaf_23

Silverleaf_24

Silverleaf_25
(lysin A)

Silverleaf_26
(lysin B)

Silverleaf_27

Silverleaf_28

Silverleaf_29

Silverleaf_30
(NrdH-like glutaredoxin)

Silverleaf_31

Silverleaf_32
(nucleotidyl transferase)

Silverleaf_33
(Lsr2-like DNA bridging protein)

Silverleaf_34

Silverleaf_35
(integrase)

Silverleaf_36

Silverleaf_37

Silverleaf_38
(Cro protein)

Silverleaf_39
(excise)

Silverleaf_40

Silverleaf_41

Silverleaf_42

Silverleaf_43

Silverleaf_44

Silverleaf_45
(esterase)

Silverleaf_46

Silverleaf_47

Silverleaf_48
(band-7-like membrane protein)

Silverleaf_49

Silverleaf_50

Silverleaf_51

Silverleaf_52

Silverleaf_53

Silverleaf_54

Silverleaf_55

Silverleaf_56

Silverleaf_57

Silverleaf_58

Silverleaf_59
(antirepressor)

Silverleaf_60
(ERF family ssDNA binding protein)

Silverleaf_61

Silverleaf_62

Silverleaf_63

Silverleaf_64

Silverleaf_66
(HNH endonuclease)

Silverleaf_65

Silverleaf_66
(DnaB-like dsDNA helicase)

Silverleaf_67
(WhiB family transcription factor)

Silverleaf_68

Silverleaf_69

Silverleaf_70
(phosphoribosyl transferase)

Silverleaf_71
(nicotinate ribosyltransferase)

Silverleaf_72

Silverleaf_73
(polynucleotide kinase)

Silverleaf_74

Silverleaf_75
(RusA-like resolvase)

Silverleaf_76
(WhiB family transcription factor)

Silverleaf_77
(WhiB family transcription factor)

Silverleaf_78

Silverleaf_79

Silverleaf_80
(ssDNA binding protein)

Silverleaf_81

Silverleaf_82

Silverleaf_83

Silverleaf_84
(RNA ligase)

Silverleaf_85

Silverleaf_86

Silverleaf_87
(helix-turn-helix DNA-binding domain protein)

Silverleaf_88
(HNH endonuclease)

Silverleaf_89

Silverleaf_90

Silverleaf_91
(DnaQ-like exonuclease)

Silverleaf_92

Silverleaf_93

Silverleaf_94
(HNH endonuclease)

Silverleaf_95

Silverleaf_96

Silverleaf_97

Silverleaf_98

Silverleaf_101

Silverleaf_107

Silverleaf_108

Silverleaf_110

Silverleaf_113

Silverleaf_114

Silverleaf_115

Silverleaf_116

Silverleaf_117

Silverleaf_118

Silverleaf_121

Silverleaf_119

Silverleaf_120

Silverleaf_121

Silverleaf_122

Silverleaf_123
(AAA-ATPase)

Silverleaf_124

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK494092
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 7.0x10^10 PFU/ML
Date of SEA Lysate Titering Jun 28, 2017
Pitt Freezer Box# 72
Pitt Freezer Box Grid# B11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload