Mycobacterium phage Wyatt2
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Detailed Information for Phage Wyatt2
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKayla Hays and KC Bobo
Year Found2016
Location FoundCorpus Christi, TX USA
Finding InstitutionDel Mar College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates27.77 N, 97.405833 W Map
Discovery NotesThe soil was found outside of a flower shop. The soil was very moist on the day of discovery.
Naming NotesThe name comes from both of our names being Wyatt. We originally wanted it to be Wyatt squared but it was not possible on the system.
Wyatt2 was discovered by Kayla Hays and KC Bobo. However, Wyatt Hooks and Wyatt Mitchell adopted and characterized the phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 5, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2260
Genome length (bp)74010
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Lysogeny NotesSmaller plaques with a high quantity. Clear edges on plaques. Lytic life cycle.
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesGenerally the plaques are 2mm in diameter, their shape are round, and they are fairly concentrated. They have a lytic life cycle. Typically a 18-20 hour incubation time.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Wyatt2_1

Wyatt2_2
(terminase, small subunit)

Wyatt2_3

Wyatt2_4
(terminase, large subunit)

Wyatt2_5
(portal protein)

Wyatt2_6
(capsid maturation protease)

Wyatt2_7
(scaffolding protein)

Wyatt2_8
(major capsid protein)

Wyatt2_9
(head-to-tail adaptor)

Wyatt2_10
(head-to-tail stopper)

Wyatt2_11

Wyatt2_12
(tail terminator)

Wyatt2_13
(major tail protein)

Wyatt2_14
(tail assembly chaperone)

Wyatt2_15
(tail assembly chaperone)

Wyatt2_16
(tape measure protein)

Wyatt2_17
(minor tail protein)

Wyatt2_18
(minor tail protein)

Wyatt2_19

Wyatt2_20
(minor tail protein)

Wyatt2_21
(minor tail protein)

Wyatt2_22

Wyatt2_23

Wyatt2_24

Wyatt2_25
(lysin A)

Wyatt2_26
(lysin B)

Wyatt2_27

Wyatt2_28
(membrane protein)

Wyatt2_29
(membrane protein)

Wyatt2_30
(glutaredoxin)

Wyatt2_31

Wyatt2_32
(Nucleotidyl transferase)

Wyatt2_33
(Lsr2-like DNA bridging protein)

Wyatt2_34

Wyatt2_35

Wyatt2_36
(tyrosine integrase)

Wyatt2_37
(membrane protein)

Wyatt2_38
(immunity repressor)

Wyatt2_39
(Cro (control of repressor s operator))

Wyatt2_40
(excise)

Wyatt2_41

Wyatt2_42

Wyatt2_43

Wyatt2_44

Wyatt2_45

Wyatt2_46
(metallophosphoesterase)

Wyatt2_47

Wyatt2_48

Wyatt2_49

Wyatt2_50

Wyatt2_51

Wyatt2_52

Wyatt2_53

Wyatt2_54

Wyatt2_55

Wyatt2_56

Wyatt2_57

Wyatt2_58

Wyatt2_59

Wyatt2_60
(Cas4 family exonuclease)

Wyatt2_61
(antirepressor)

Wyatt2_62
(ssDNA binding protein, ERF family)

Wyatt2_63

Wyatt2_64

Wyatt2_65

Wyatt2_66
(membrane protein)

Wyatt2_67

Wyatt2_68

Wyatt2_69
(DnaB-like dsDNA helicase)

Wyatt2_70
(WhiB family transcription factor)

Wyatt2_71

Wyatt2_72

Wyatt2_73
(phosphoribosyl transferase)

Wyatt2_74
(nicotinate ribosyltransferase)

Wyatt2_75

Wyatt2_76
(polynucleotide kinase)

Wyatt2_77

Wyatt2_78
(RusA-like resolvase (endonuclease))

Wyatt2_79
(WhiB family transcription factor)

Wyatt2_80
(WhiB family transcription factor)

Wyatt2_81

Wyatt2_82

Wyatt2_83
(ssDNA binding protein)

Wyatt2_84
(toxin in toxin/antitoxin system, HicA-like)

Wyatt2_85

Wyatt2_86

Wyatt2_87
(RNA ligase)

Wyatt2_88

Wyatt2_89

Wyatt2_90

Wyatt2_91

Wyatt2_92
(HNH endonuclease)

Wyatt2_93

Wyatt2_94

Wyatt2_95
(DnaQ-like (DNA polymerase III subunit))

Wyatt2_96

Wyatt2_97

Wyatt2_98
(endonuclease VII)

Wyatt2_99
(HNH endonuclease)

Wyatt2_100

Wyatt2_101

Wyatt2_102

Wyatt2_103

Wyatt2_106

Wyatt2_112

Wyatt2_113

Wyatt2_114

Wyatt2_116

Wyatt2_117

Wyatt2_118

Wyatt2_119

Wyatt2_120

Wyatt2_121

Wyatt2_122

Wyatt2_123

Wyatt2_124

Wyatt2_125

Wyatt2_126

Wyatt2_127

Wyatt2_128

Wyatt2_129
(AAA-ATPase)

Wyatt2_130

Wyatt2_131

Wyatt2_132

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN586018
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 34
Pitt Freezer Box Grid# H9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload