Mycobacterium phage DirkDirk
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Detailed Information for Phage DirkDirk
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMadison Risi, Lisa Mitchell, D. Nicke, A. Tubman
Year Found2017
Location FoundBoulder, CO United States
Finding InstitutionUniversity of Colorado at Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.001 N, 105.27 W Map
Discovery NotesFlower pot in front of Norlin Library on CU Boulder campus containing moist soil, mulch, and some trash. Collected by Daria Nicke.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 1, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage689
Genome length (bp)68021
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.7%
Sequencing NotesThis phage was sequenced as part of a mixed DOGEMS sample. The sequence of this genome was confirmed to belong to this phage by PCR.
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusBeing Annotated (Expected completion by 5/15/2019)
Plaque NotesTurbid, 2-5 mm in diameter
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 62
Pitt Freezer Box Grid# F4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload