Mycobacterium phage Winky
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Detailed Information for Phage Winky
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByOlivia Martino
Year Found2011
Location FoundLanghorne, PA USA
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.164913 N, 74.972458 W Map
Discovery NotesThe soil was collected from a moist flowerbed in Langhorne, a suburb surrounding Philadelphia, approximately 2 feet from a house and 5 cm deep.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 21, 2012
Sequencing FacilityVirginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method454 Pyrosequencing
Genome length (bp)76653
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques are large, 1-3mm with very clear centers surrounded by a cloudy ring - “bulls eye” pattern
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Winky_1

Winky_2
(terminase small subunit)

Winky_3

Winky_4
(terminase large subunit)

Winky_5
(portal protein)

Winky_6
(capsid maturation protease)

Winky_7
(scaffolding protein)

Winky_8
(major capsid protein)

Winky_9
(head-to-tail adaptor)

Winky_10
(head-to-tail stopper)

Winky_11

Winky_12
(tail terminator)

Winky_13
(major tail subunit)

Winky_14
(tail assembly chaperone)

Winky_15
(tail assembly chaperone)

Winky_16
(tapemeasure)

Winky_17
(minor tail protein)

Winky_18
(minor tail protein)

Winky_19
(minor tail protein)

Winky_20
(minor tail protein)

Winky_21

Winky_22

Winky_23

Winky_24
(minor tail protein)

Winky_25

Winky_26

Winky_27
(lysin A)

Winky_28
(lysin B)

Winky_29
(holin)

Winky_30

Winky_31

Winky_32
(NrdH-like protein)

Winky_33

Winky_34

Winky_35
(nucleotidyl transferase NrdC-like protein)

Winky_36
(Lsr2)

Winky_37

Winky_38
(integrase (y-int))

Winky_39
(HTH DNA binding domain protein)

Winky_40
(immunity repressor)

Winky_41

Winky_42

Winky_43

Winky_44

Winky_45

Winky_46

Winky_47
(esterase)

Winky_48

Winky_49

Winky_50

Winky_51

Winky_52

Winky_53

Winky_54

Winky_55

Winky_56

Winky_57

Winky_58

Winky_59

Winky_60

Winky_61

Winky_62

Winky_63

Winky_64
(Erf)

Winky_65

Winky_66

Winky_67
(HNH endonuclease domain protein)

Winky_68

Winky_69

Winky_70
(DnaB-like helicase)

Winky_71

Winky_72

Winky_73

Winky_74
(ribose phosphate kinase)

Winky_75
(nicotinate phosphoribosyltransferase)

Winky_76
(polynucleotide kinase)

Winky_77

Winky_78

Winky_79

Winky_80
(RusA)

Winky_81
(WhiB)

Winky_82
(WhiB)

Winky_83

Winky_84

Winky_85
(ssDNA binding protein)

Winky_86

Winky_87

Winky_88
(RNA ligase)

Winky_89

Winky_90
(ClpP-like protease)

Winky_91

Winky_92

Winky_93

Winky_94

Winky_95
(HNH endonuclease domain protein)

Winky_96

Winky_97

Winky_98
(DnaQ-like protein)

Winky_99

Winky_100

Winky_101

Winky_102

Winky_103

Winky_104

Winky_106

Winky_110

Winky_111

Winky_117

Winky_118

Winky_120

Winky_121

Winky_123

Winky_125

Winky_126

Winky_127

Winky_128

Winky_129

Winky_130

Winky_131

Winky_132

Winky_133

Winky_134

Winky_135

Winky_136

Winky_137

Winky_138

Winky_139

Winky_140

Winky_141

Winky_142

Winky_143

Winky_144

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKC661276
Refseq NumberNone yet
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 7.9e10
Pitt Freezer Box# 27
Pitt Freezer Box Grid# 10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload