Mycobacterium phage MAckerman
Know something about this phage that we don't? Modify its data.
Detailed Information for Phage MAckerman
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDominic Picone and Riley Skinner
Year Found2022
Location FoundEast Greenwich, RI United States
Finding InstitutionMerrimack College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature20°C
GPS Coordinates41.66054 N, 71.46926 W Map
Discovery Noteshe temperature was 68F and humidity 64% at time of dig. Grass was dug up next to the foundation of the house. Location was under an air vent that is connected to the washer and dryer. It produces heat and lint onto the grass outside where the dirt was taken. Grass was ripped out with gloves on until dirt was seen. Dirt was scooped up with fingers into the 15 mL conical tube.
Naming NotesNamed after a character in the popular anime series Attack on Titan.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 14, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage358
Genome length (bp)73874
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionMerrimack College
Annotation StatusBeing Annotated (Expected completion by 5/1/2023)
Plaque NotesIn generation 1 our plate was filled with many plaques all about 0.1 cm in diameter and with clean edge margins. Majority of the plaques were not foggy, including the one used for generation 2. The plaque selected by our group to be concentrated more was a large plaque compared to the rest, a little over 0.1 cm in size with very clean edge margins. The second generation plate had less plaques on it then the first generation, these plaques were roughly the same size, 0.1 cm, with relatively clean edges. These plaques were not as clear as generation 1 and seemed a bit more foggy. The plaque from generation 2 that was selected to be concentrated was the largest out of them all, approximately 0.2 cm. This plaque had nice clean edge margins but was much foggier than generation 1. Our group's generation 3 plate contained a lot of larger plaques
compared to the rest. These plaques were roughly 3mm in size, most with clean edge margins but still foggier than our generation 1 plaques. The plaque selected from generation 3 to be
concentrated further was the largest from the plate, about 0.35 cm in diameter. This plaque had nice clean edges but was slightly foggy and not as clear as some of the other plaques.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

MAckerman_Draft_1

MAckerman_Draft_2

MAckerman_Draft_3

MAckerman_Draft_4

MAckerman_Draft_5

MAckerman_Draft_6

MAckerman_Draft_7

MAckerman_Draft_8

MAckerman_Draft_9

MAckerman_Draft_10

MAckerman_Draft_11

MAckerman_Draft_12

MAckerman_Draft_13

MAckerman_Draft_14

MAckerman_Draft_15

MAckerman_Draft_16

MAckerman_Draft_17

MAckerman_Draft_18

MAckerman_Draft_19

MAckerman_Draft_20

MAckerman_Draft_21

MAckerman_Draft_22

MAckerman_Draft_23

MAckerman_Draft_24

MAckerman_Draft_25

MAckerman_Draft_26

MAckerman_Draft_27

MAckerman_Draft_28

MAckerman_Draft_29

MAckerman_Draft_30

MAckerman_Draft_31

MAckerman_Draft_32

MAckerman_Draft_33

MAckerman_Draft_34

MAckerman_Draft_35

MAckerman_Draft_36

MAckerman_Draft_37

MAckerman_Draft_38

MAckerman_Draft_39

MAckerman_Draft_40

MAckerman_Draft_41

MAckerman_Draft_42

MAckerman_Draft_43

MAckerman_Draft_44

MAckerman_Draft_45

MAckerman_Draft_46

MAckerman_Draft_47

MAckerman_Draft_48

MAckerman_Draft_49

MAckerman_Draft_50

MAckerman_Draft_51

MAckerman_Draft_52

MAckerman_Draft_53

MAckerman_Draft_54

MAckerman_Draft_55

MAckerman_Draft_56

MAckerman_Draft_57

MAckerman_Draft_58

MAckerman_Draft_59

MAckerman_Draft_60

MAckerman_Draft_61

MAckerman_Draft_62

MAckerman_Draft_63

MAckerman_Draft_64

MAckerman_Draft_65

MAckerman_Draft_66

MAckerman_Draft_67

MAckerman_Draft_68

MAckerman_Draft_69

MAckerman_Draft_70

MAckerman_Draft_71

MAckerman_Draft_72

MAckerman_Draft_73

MAckerman_Draft_74

MAckerman_Draft_75

MAckerman_Draft_76

MAckerman_Draft_77

MAckerman_Draft_78

MAckerman_Draft_79

MAckerman_Draft_80

MAckerman_Draft_81

MAckerman_Draft_82

MAckerman_Draft_83

MAckerman_Draft_84

MAckerman_Draft_85

MAckerman_Draft_86

MAckerman_Draft_87

MAckerman_Draft_88

MAckerman_Draft_89

MAckerman_Draft_90

MAckerman_Draft_91

MAckerman_Draft_92

MAckerman_Draft_93

MAckerman_Draft_94

MAckerman_Draft_95

MAckerman_Draft_96

MAckerman_Draft_97

MAckerman_Draft_98

MAckerman_Draft_99

MAckerman_Draft_102

MAckerman_Draft_106

MAckerman_Draft_107

MAckerman_Draft_109

MAckerman_Draft_110

MAckerman_Draft_112

MAckerman_Draft_114

MAckerman_Draft_115

MAckerman_Draft_116

MAckerman_Draft_117

MAckerman_Draft_118

MAckerman_Draft_119

MAckerman_Draft_120

MAckerman_Draft_121

MAckerman_Draft_122

MAckerman_Draft_123

MAckerman_Draft_124

MAckerman_Draft_125

MAckerman_Draft_126

MAckerman_Draft_127

MAckerman_Draft_128

MAckerman_Draft_129

MAckerman_Draft_130

Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload