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Mycobacterium phage Enceladus
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Detailed Information for Phage Enceladus
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByDani Buchheister & Hannah Sotiroff
Year Found2020
Location FoundBoulder, CO USA
Finding InstitutionUniversity of Colorado at Boulder
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature-8°C
GPS Coordinates40.009133 N, 105.269789 W Map
Discovery NotesFound near a classroom building in a landscaped area under a tree. Ground cover was mostly mulch with some bushes and shrubs.
Naming NotesOur phage is named after a giant in Greek mythology. Enceladus is also the name of one of Saturn's icy moons, a promising site for the search for life in the solar system. It's Dani's favorite moon, and it fits since our phage was taken from the soil on a cold and snowy day.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 22, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage427
Genome length (bp)66420
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Colorado at Boulder
Annotation StatusIn GenBank
Plaque NotesPlaques have clear edges and range from 3-5 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Enceladus_1

Enceladus_2
(terminase small subunit)

Enceladus_3

Enceladus_4
(terminase large subunit)

Enceladus_5
(portal protein)

Enceladus_6

Enceladus_7
(scaffolding protein)

Enceladus_8
(major capsid protein)

Enceladus_9
(head-to-tail adaptor)

Enceladus_10
(head-to-tail stopper)

Enceladus_11

Enceladus_12
(tail terminator)

Enceladus_13
(major tail protein)

Enceladus_14
(tail assembly chaperone)

Enceladus_15
(tail assembly chaperone)

Enceladus_16
(tape measure protein)

Enceladus_17
(minor tail protein)

Enceladus_18
(minor tail protein)

Enceladus_19

Enceladus_20
(minor tail protein)

Enceladus_21
(minor tail protein)

Enceladus_22

Enceladus_23

Enceladus_24

Enceladus_25
(lysin A)

Enceladus_26
(Lysin B)

Enceladus_27

Enceladus_28

Enceladus_29

Enceladus_30
(NrdH-like glutaredoxin)

Enceladus_31

Enceladus_32
(nucleotidyltransferase)

Enceladus_33
(Lsr2-like DNA bridging protein)

Enceladus_34

Enceladus_35
(tyrosine integrase)

Enceladus_36

Enceladus_37
(immunity repressor)

Enceladus_38
(Cro protein)

Enceladus_39
(excise)

Enceladus_40

Enceladus_41

Enceladus_42

Enceladus_43

Enceladus_44

Enceladus_45
(esterase)

Enceladus_46

Enceladus_47

Enceladus_48
(Membrane protein Band 7-like)

Enceladus_49

Enceladus_50

Enceladus_51

Enceladus_52
(Ro-like RNA Binding Protein)

Enceladus_53

Enceladus_54

Enceladus_55

Enceladus_56

Enceladus_57
(Cas4 Family Endonuclease)

Enceladus_58
(antirepressor)

Enceladus_59
(ssDNA binding protein, ERF family)

Enceladus_60

Enceladus_61

Enceladus_62

Enceladus_63
(HNH endonuclease)

Enceladus_64

Enceladus_65
(DNAB-like dsDNA helicase)

Enceladus_66
(WhiB family transcription factor)

Enceladus_67

Enceladus_68

Enceladus_69

Enceladus_70
(Nicotinate ribosyltransferase)

Enceladus_71

Enceladus_72
(polynucleotide kinase)

Enceladus_73

Enceladus_74
(RusA-like resolvase)

Enceladus_75
(WhiB family transcription factor)

Enceladus_76
(WhiB family transcription factor)

Enceladus_77

Enceladus_78

Enceladus_79
(ssDNA binding protein)

Enceladus_80

Enceladus_81

Enceladus_82
(DprA-like DNA processing chain A)

Enceladus_83
(RNA Ligase)

Enceladus_84

Enceladus_85

Enceladus_86

Enceladus_87

Enceladus_88
(HNH Endonuclease)

Enceladus_89

Enceladus_90

Enceladus_91
(Dna Q-like (DNA polymerase III subunit))

Enceladus_92

Enceladus_93

Enceladus_94
(HNH Endonuclease)

Enceladus_95

Enceladus_96

Enceladus_97

Enceladus_98

Enceladus_99

Enceladus_102

Enceladus_108

Enceladus_109

Enceladus_111

Enceladus_113

Enceladus_114

Enceladus_115
(AAA-ATPase)

Enceladus_116

Enceladus_117

Enceladus_118

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMZ958746
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 126
Pitt Freezer Box Grid# G4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload