Mycobacterium phage Rose5
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Detailed Information for Phage Rose5
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJoel Winthrope
Year Found2019
Location FoundCorpus Christi, TX United States of America
Finding InstitutionDel Mar College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature29°C
GPS Coordinates27.65 N, 97.41 W Map
Discovery NotesGathered from Botanical gardens.
Soil sample was taken next to cinco de mayo roses.
Soil temperature was 29.4 degrees celsius.
Ambient temperature was 29.8 degrees celsius.
Ambient humidity was 100%; it had rained approximately an hour before collection.
Closest body of water is the Oso river.
Naming Notesnamed because sample was taken from a rose garden.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 14, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage643
Genome length (bp)73949
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGAACGGCC
GC Content58.7%
Sequencing NotesThe sample that was sequenced seemed to be contaminated with a low level (~5%) of phage Nanosmite.
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL1
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionWashington State University
Annotation StatusIn GenBank
Plaque NotesAvg. plaque diameter is 1mm
plaques are very clear

Lytic life cycle
seems to be very aggressive
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Rose5_1

Rose5_2
(terminase small subunit)

Rose5_3

Rose5_4
(terminase large subunit)

Rose5_5
(portal protein)

Rose5_6
(MuF-like minor capsid protein)

Rose5_7
(scaffolding protein)

Rose5_8
(major capsid protein)

Rose5_9
(head-to-tail adaptor)

Rose5_10
(head-to-tail stopper)

Rose5_11

Rose5_12
(tail terminator)

Rose5_13
(major tail protein)

Rose5_14
(tail assembly chaperone)

Rose5_15
(tail assembly chaperone)

Rose5_16
(tape measure protein)

Rose5_17
(minor tail protein)

Rose5_18
(minor tail protein)

Rose5_19

Rose5_20
(minor tail protein)

Rose5_21
(minor tail protein)

Rose5_22

Rose5_23

Rose5_24

Rose5_25
(lysin A)

Rose5_26
(lysin B)

Rose5_27

Rose5_28

Rose5_29

Rose5_30
(NrdH-like glutaredoxin)

Rose5_31

Rose5_32
(nucleotidyl transferase)

Rose5_33
(Lsr2-like DNA bridging protein)

Rose5_34

Rose5_35
(tyrosine integrase)

Rose5_36

Rose5_37
(immunity repressor)

Rose5_38
(Cro protein)

Rose5_39
(excise)

Rose5_40

Rose5_41

Rose5_42

Rose5_44

Rose5_45
(esterase)

Rose5_46

Rose5_47

Rose5_48
(band-7-like membrane protein)

Rose5_49

Rose5_50

Rose5_51

Rose5_52

Rose5_53
(Ro-like RNA binding protein)

Rose5_54

Rose5_55

Rose5_56

Rose5_57

Rose5_58

Rose5_59
(exonuclease)

Rose5_60
(antirepressor)

Rose5_61
(ERF family ssDNA binding protein)

Rose5_62

Rose5_63

Rose5_64

Rose5_65
(HNH endonuclease)

Rose5_66
(HNH endonuclease)

Rose5_67

Rose5_68
(DnaB-like dsDNA helicase)

Rose5_69
(WhiB family transcription factor)

Rose5_70

Rose5_71

Rose5_72
(pyrophosphate kinase)

Rose5_73
(nicotinate ribosyltransferase)

Rose5_74

Rose5_75
(polynucleotide kinase)

Rose5_76

Rose5_77
(RusA-like resolvase)

Rose5_78
(WhiB family transcription factor)

Rose5_79
(WhiB family transcription factor)

Rose5_80

Rose5_81

Rose5_82

Rose5_83
(ssDNA binding protein)

Rose5_84
(HicA-like toxin)

Rose5_85

Rose5_86
(DprA-like DNA processing chain A)

Rose5_87
(RNA ligase)

Rose5_88

Rose5_89

Rose5_90

Rose5_91
(helix-turn-helix DNA binding protein)

Rose5_92
(HNH endonuclease)

Rose5_93

Rose5_94

Rose5_95
(DnaQ-like DNA polymerase III subunit)

Rose5_96

Rose5_97

Rose5_98

Rose5_99
(HNH endonuclease)

Rose5_100

Rose5_101

Rose5_102

Rose5_103

Rose5_106

Rose5_112

Rose5_113

Rose5_115

Rose5_117

Rose5_118

Rose5_119

Rose5_120

Rose5_121

Rose5_122

Rose5_123

Rose5_124
(helix-turn-helix DNA binding domain protein)

Rose5_125

Rose5_126

Rose5_127

Rose5_128

Rose5_129

Rose5_130
(AAA-ATPase)

Rose5_131

Rose5_132

Rose5_133

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW822149
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 101
Pitt Freezer Box Grid# A1
Available Files
GenBank File for PhameratorDownload