Mycobacterium phage Nicholas

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Detailed Information for Phage Nicholas
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByLydia Bricker, Sierra Hobbs, and Michelle Lapak
Year Found2016
Location FoundGulf Breeze, FL United States of America
Finding InstitutionUniversity of West Florida
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS CoordinatesUnavailable
Naming NotesWe named our phage as a tribute to the great actor of our time, Nicolas Cage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1885
Genome length (bp)70413
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.2%
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterL
SubclusterL3
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Nicholas_1

Nicholas_2
(terminase small subunit)

Nicholas_3

Nicholas_4

Nicholas_5
(terminase large subunit)

Nicholas_6
(portal protein)

Nicholas_7
(capsid maturation protease)

Nicholas_8
(scaffolding protein)

Nicholas_9
(major capsid protein)

Nicholas_10
(head-to-tail connector protein)

Nicholas_11
(head-to-tail connector protein)

Nicholas_12
(head-to-tail connector protein)

Nicholas_13
(head-to-tail connector protein)

Nicholas_14
(major tail protein)

Nicholas_15
(tail assembly chaperone)

Nicholas_16
(tail assembly chaperone)

Nicholas_17
(tape measure protein)

Nicholas_18
(minor tail protein)

Nicholas_19
(minor tail protein)

Nicholas_20
(minor tail protein)

Nicholas_21
(minor tail protein)

Nicholas_22
(minor tail protein)

Nicholas_23

Nicholas_24

Nicholas_25

Nicholas_26
(lysin A)

Nicholas_27
(holin)

Nicholas_28
(lysin B)

Nicholas_29

Nicholas_30

Nicholas_31
(glutaredoxin)

Nicholas_32

Nicholas_33
(nucleotidyltransferase)

Nicholas_34
(DNA binding protein)

Nicholas_35

Nicholas_36
(integrase)

Nicholas_37

Nicholas_38
(immunity repressor)

Nicholas_39

Nicholas_40
(DNA binding protein)

Nicholas_41

Nicholas_42

Nicholas_43

Nicholas_44

Nicholas_45

Nicholas_46

Nicholas_47

Nicholas_48
(membrane protein)

Nicholas_50

Nicholas_51

Nicholas_52

Nicholas_53

Nicholas_54
(RNA binding protein)

Nicholas_55

Nicholas_56

Nicholas_57

Nicholas_58

Nicholas_59

Nicholas_60

Nicholas_61
(ERF family ssDNA binding protein)

Nicholas_62
(ERF family ssDNA binding protein)

Nicholas_63
(DNA binding protein)

Nicholas_64

Nicholas_65

Nicholas_66

Nicholas_67
(DNA helicase)

Nicholas_68

Nicholas_69

Nicholas_70

Nicholas_71

Nicholas_72
(ribose-phosphate pyrophosphokinase)

Nicholas_73
(ribose-phosphate pyrophosphokinase)

Nicholas_74
(polynucleotide kinase)

Nicholas_75

Nicholas_76
(resolvase)

Nicholas_77
(WhiB family transcription factor)

Nicholas_78
(WhiB family transcription factor)

Nicholas_79

Nicholas_80

Nicholas_81
(ssDNA binding protein)

Nicholas_82

Nicholas_83

Nicholas_84
(RNA ligase)

Nicholas_85
(protease)

Nicholas_86

Nicholas_87

Nicholas_88

Nicholas_89

Nicholas_90
(HNH endonuclease)

Nicholas_91

Nicholas_92

Nicholas_93
(DNA polymerase III subunit)

Nicholas_94

Nicholas_95

Nicholas_96

Nicholas_97

Nicholas_98

Nicholas_99

Nicholas_100

Nicholas_102

Nicholas_104

Nicholas_108

Nicholas_111

Nicholas_112

Nicholas_115

Nicholas_116

Nicholas_117

Nicholas_118

Nicholas_119

Nicholas_120

Nicholas_121

Nicholas_122

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF140421
Refseq NumberNone yet
Published in PaperChung HM et al 2017, Genome Announcements: Complete Genome Sequences of Mycobacteriophages Clautastrophe, Kingsolomon, Krypton555, and Nicholas
Archiving Info
Archiving status Archived
Pitt Freezer Box# 43
Pitt Freezer Box Grid# C9
Available Files
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload