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Mycobacterium phage MiniMac
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Detailed Information for Phage MiniMac
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCaitlin McCaffrey
Year Found2018
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates29.81808 S, 30.94255 E Map
Discovery NotesMiniMac was discovered beneath a small tree in a flowerbed found in the car park of the finance and administration department (above the cafeteria) at the University of KwaZulu-Natal. The soil sample appeared light in colour and relatively dry which could be owing to the lack of rain in recent weeks. The phage plaques were visible on the agar plates approximately 24-36 hours after plating, and appeared first following a spot test of the environmental sample's filtrate.
Naming NotesThe name of this mycobacteriophage originated from my surname being McCaffrey. My mother - Sarah McCaffrey - is known by her close friends as Mac, this filtered down to my nickname being little Mac. MiniMac, therefore, being the logical name for the newest and smallest addition to the family.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 9, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage714
Genome length (bp)70064
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL3
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Kwazulu-Natal
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

MiniMac_1

MiniMac_2
(terminase small subunit)

MiniMac_3

MiniMac_4

MiniMac_5
(terminase large subunit)

MiniMac_6
(portal protein)

MiniMac_7
(capsid maturation protease)

MiniMac_8
(scaffolding protein)

MiniMac_9
(major capsid protein)

MiniMac_10
(head-to-tail adaptor)

MiniMac_11
(head-to-tail stopper)

MiniMac_12

MiniMac_13
(tail terminator)

MiniMac_14
(major tail protein)

MiniMac_15
(tail assembly chaperone)

MiniMac_16
(tail assembly chaperone)

MiniMac_17
(tape measure protein)

MiniMac_18
(minor tail protein)

MiniMac_19
(minor tail protein)

MiniMac_20

MiniMac_21
(minor tail protein)

MiniMac_22
(minor tail protein)

MiniMac_23

MiniMac_24

MiniMac_25

MiniMac_26
(lysin A)

MiniMac_27
(holin)

MiniMac_28
(lysin B)

MiniMac_29
(glycoside hydrolase)

MiniMac_30

MiniMac_31
(NrdH-like glutaredoxin)

MiniMac_32

MiniMac_33
(nucleotidyl transferase)

MiniMac_34
(Lsr2-like DNA bridging protein)

MiniMac_35

MiniMac_36
(tyrosine integrase)

MiniMac_37

MiniMac_38
(immunity repressor)

MiniMac_39
(Cro protein)

MiniMac_40
(excise)

MiniMac_41

MiniMac_42

MiniMac_43

MiniMac_44

MiniMac_45

MiniMac_46
(metallophosphoesterase)

MiniMac_47

MiniMac_48

MiniMac_49

MiniMac_50
(band-7-like membrane protein)

MiniMac_52

MiniMac_53

MiniMac_54

MiniMac_55

MiniMac_56

MiniMac_57
(Ro-like RNA binding protein)

MiniMac_58

MiniMac_59

MiniMac_60

MiniMac_61

MiniMac_62

MiniMac_63

MiniMac_64
(Cas4 family exonuclease)

MiniMac_65
(ERF family ssDNA binding protein)

MiniMac_66

MiniMac_67
(ParB-like nuclease domain protein)

MiniMac_68

MiniMac_69
(HNH endonuclease)

MiniMac_70

MiniMac_71

MiniMac_72

MiniMac_73
(DNA helicase)

MiniMac_74
(WhiB family transcription factor)

MiniMac_75

MiniMac_76

MiniMac_77

MiniMac_78
(phosphoribosyl transferase)

MiniMac_79
(nicotinate ribosyltransferase)

MiniMac_80
(polynucleotide kinase)

MiniMac_81

MiniMac_82
(RusA-like resolvase)

MiniMac_83
(WhiB family transcription factor)

MiniMac_84
(WhiB family transcription factor)

MiniMac_85

MiniMac_86

MiniMac_87
(ssDNA binding protein)

MiniMac_88
(HicA-like toxin)

MiniMac_89

MiniMac_90
(RNA ligase)

MiniMac_91
(ClpP-like protease)

MiniMac_92

MiniMac_93

MiniMac_94

MiniMac_95
(helix-turn-helix DNA binding domain protein)

MiniMac_96
(HNH endonuclease)

MiniMac_97

MiniMac_98

MiniMac_99
(DnaQ-like DNA polymerase III)

MiniMac_100

MiniMac_101

MiniMac_102
(HNH endonuclease)

MiniMac_103

MiniMac_104

MiniMac_105

MiniMac_106

MiniMac_108

MiniMac_110

MiniMac_115

MiniMac_118
(helix-turn-helix DNA binding domain protein)

MiniMac_119

MiniMac_120

MiniMac_123

MiniMac_124
(ADP-ribosyltransferase)

MiniMac_125

MiniMac_126

MiniMac_127

MiniMac_128

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT889391
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 96
Pitt Freezer Box Grid# C10
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload