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Mycobacterium phage MiniMac
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Detailed Information for Phage MiniMac
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCaitlin McCaffrey
Year Found2018
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates29.81808 S, 30.94255 E Map
Discovery NotesMiniMac was discovered beneath a small tree in a flowerbed found in the car park of the finance and administration department (above the cafeteria) at the University of KwaZulu-Natal. The soil sample appeared light in colour and relatively dry which could be owing to the lack of rain in recent weeks. The phage plaques were visible on the agar plates approximately 24-36 hours after plating, and appeared first following a spot test of the environmental sample's filtrate.
Naming NotesThe name of this mycobacteriophage originated from my surname being McCaffrey. My mother - Sarah McCaffrey - is known by her close friends as Mac, this filtered down to my nickname being little Mac. MiniMac, therefore, being the logical name for the newest and smallest addition to the family.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 9, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage714
Genome length (bp)70064
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content59.2%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL3
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Kwazulu-Natal
Annotation StatusBeing Annotated (Expected completion by 1/9/2020)
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload