| Detailed Information for Phage Attis |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Duplicates of this Phage | Fiorenza |
| Found By | Melanie Umbaugh |
| Year Found | 2015 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 31°C |
| GPS Coordinates | 40.443889 N, 79.957222 W Map |
| Discovery Notes | Soil was collected from a flower garden of marigolds outside of Thackeray Hall at the University of Pittsburgh. The marigolds that were near the soil collection site were wilting and decaying. The sample was collected 2 to 3 inches from the surface and the garden had been watered approximately an hour before collection. It was 87*F. |
| Naming Notes | The name Attis comes from Greek mythology. Attis was the god of rebirth and resurrection. The terms "rebirth" and "resurrection" seemed to describe bacteriophage activity: infecting and inducing lysis in a host cell in order to produce new phage particles. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 12, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 949 |
| Genome length (bp) | 47881 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TACCAGGGGGA |
| GC Content | 66.8% |
| Sequencing Notes | small amount of reads have deletion from 23,973 bp to 24059 bp, total deletion 87 bp inclusive |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Dual plaque morphology has been observed. Small, pinprick plaques are 1 mm and clear. Other larger plaques are 3 mm and are surrounded by a cloudy ring. |
| Number of Genes | 74 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU963247 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
2 |
| Pitt Freezer Box Grid# |
C3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Final DNAMaster File | Download |