| Detailed Information for Phage Bosnia |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Lejla Suljevic |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 27°C |
| GPS Coordinates | 40.442102 N, 79.965586 W Map |
| Discovery Notes | The sample was collected in Pittsburgh, PA in front of a house on Robinson St. The soil was rather loose, dark, wet, and contained woodchips. When collected, the weather was 81°F at around 9:00am. |
| Naming Notes | The name was given as a nod to the country that I was born in, Bosnia and Herzegovina. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 25, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1772 |
| Genome length (bp) | 54546 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Alliance University (formerly Nyack College) |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques are consistently small, clear, and circular, indicating that there is only one type of phage. The plaques were less than 2 mm in length. |
| Morphotype | Siphoviridae |
| Number of Genes | 82 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT952844 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.33x10^10 |
| Pitt Freezer Box# |
89 |
| Pitt Freezer Box Grid# |
G12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
