| Detailed Information for Phage Bromden |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Hannah E Cizauskas |
| Year Found | 2017 |
| Location Found | Westland, MI USA |
| Finding Institution | Hope College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.2896 N, 83.4289 W Map |
| Discovery Notes | Slightly moist soil from a pumpkin patch that was composted with grass clippings. |
| Naming Notes | This phage is named after the main character in One Flew Over the Cuckoo's Nest, written by Ken Kesey. Chief Bromden is the only character that escaped the insane asylum by checking himself out. The other characters either remained in the asylum or died in there. He was the one that got out, much like my phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1973 |
| Genome length (bp) | 70183 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 58.2% |
| Sequencing Notes | Despite a high level of coverage, the overhang was not present in the sequencing reads, and so it was inferred from highly similar genomes. Sanger sequencing will be attempted to cover this region. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Hope College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are circular, clear, and .5mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 118 |
| Number of tRNAs | 13 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH576973 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
50 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
