| Detailed Information for Phage Dolores |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Rebecca Cohen |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.439423 N, 79.947033 W Map |
| Discovery Notes | On 1/10 the weather forecast said a high of 50 and a low of 25. It was lightly raining at 12:20pm when I walked to Phipps Conservatory, a public garden in Pittsburgh PA. I collected a soil sample from the walkway leading into the Conservatory. The GPS coordinates of the location were 40.439423 degrees North and 79.947033 degrees West. The soil was covered in mulch and very hard. I believe it was still slightly frozen from the day before which had a high of 29 and a low of 7. The soil sample was kept in my backpack until 4:30pm lab. |
| Naming Notes | My roommate was going to be named Dolores, which was an interesting topic of discussion around the time I discovered a phage. I named this phage Dolores in hopes that she would be an interesting topic of discussion. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 791 |
| Genome length (bp) | 47336 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TGCCAAGGGGA |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Mitchell Community College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appear as a tiny, clear spots in the middle ~2mm with turbid halos surrounding them. Differences in halo sizes cause plaques to vary from ~3mm to 5 mm. |
| Number of Genes | 80 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ681517 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
55 |
| Pitt Freezer Box Grid# |
F1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
