| Detailed Information for Phage Douge |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Li-An Tsai |
| Year Found | 2021 |
| Location Found | Hsinchu, Taiwan |
| Finding Institution | Academia Sinica |
| Program | Unavailable |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 24.748236 N, 121.037344 E Map |
| Discovery Notes | The soil sample was collected under the cypress. The soil there was moist because of the shadow of the tree. Surface soil was collected and contained some leaves. |
| Naming Notes | This phage, Douge, was found in the garden of my community. Douge is my pet dog. He died in 2021 winter. So this name is to memorize Douge. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 22, 2022 |
| Sequencing Facility | Biodiversity Research Center at Academia Sinica, Taiwan |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1336 |
| Genome length (bp) | 74574 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 58.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L4 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Academia Sinica |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque is around 10 mm in diameter and with clear edge. |
| Morphotype | Siphoviridae |
| Number of Genes | 132 |
| Number of tRNAs | 12 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ184818 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
