| Detailed Information for Phage DrSeegs |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Addison Lay |
| Year Found | 2020 |
| Location Found | Lewistown, MO United States |
| Finding Institution | Culver-Stockton College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.081182 N, 91.81485 W Map |
| Discovery Notes | The soil sample was collected under a large tree, and it had been raining the night prior. A shovel was used to remove the top layer of soil in order to collect the sample |
| Naming Notes | The phage is named for the professor of the class in which it was discovered |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 22, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 210 |
| Genome length (bp) | 76652 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 58.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Culver-Stockton College |
| Annotation Status | In GenBank |
| Plaque Notes | Lytic, 1.5mm diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 128 |
| Number of tRNAs | 12 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ171373 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.7 x 10^10 pfu/mL |
| Pitt Freezer Box# |
120 |
| Pitt Freezer Box Grid# |
G11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
