| Detailed Information for Phage DuncansLeg |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Kaitlynn Burbage |
| Year Found | 2021 |
| Location Found | Conway, SC United States of America |
| Finding Institution | Coastal Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.7993 N, 79.0138 W Map |
| Discovery Notes | loamy soil, moist, plant roots, top of soil, dark brown |
| Naming Notes | Named after pet cat, Duncan, who got one of his legs amputated |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 677 |
| Genome length (bp) | 75593 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Coastal Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | medium, circular, uniform |
| Morphotype | Siphoviridae |
| Number of Genes | 126 |
| Number of tRNAs | 10 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON755181 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
135 |
| Pitt Freezer Box Grid# |
H1 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
