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Detailed Information for Phage GuuelaD
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Kysha M. Gonzalez-Montes
Year Found	2015
Location Found	Arroyo, PR 17.992600 N
Finding Institution	University of Puerto Rico at Cayey
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Unknown
Isolation Temperature	37°C
GPS Coordinates	Unavailable
Discovery Notes	84F Superficial Dark moist soil. Near sewer drain.
Naming Notes	Guuela: Spanish slang for grandmother. D: Grandmother's name begins with the letter "D"
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Mar 24, 2016
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	575
Genome length (bp)	76315
Character of genome ends	3' Sticky Overhang
Overhang Length	10 bases
Overhang Sequence	TCGATCAGCC
GC Content	58.9%
Fasta file available?	Available upon entering GPS coordinates
Characterization
Cluster	L
Subcluster	L2
Cluster Life Cycle	Temperate
Lysogeny Notes	2 purifications lysogenic.
Other Cluster Members	Click to View Acquire49 (L1) Appletree2 (L1) AvadaKedavra (L1) Calm (L1) CicholasNage (L1) DirkDirk (L1) Enceladus (L1) Halena (L1) JoeDirt (L1) LeBron (L1) MAckerman (L1) OhShagHennessy (L1) Poochiewood (L1) Rose5 (L1) Silverleaf (L1) Tyson (L1) UPIE (L1) Wamburgrxpress (L1) Wyatt2 (L1) Zaria (L1) Archie (L2) Baoshan (L2) Bazzle (L2) BigCheese (L2) BobsGarage (L2) Breezona (L2) Claus (L2) Crossroads (L2) DanBing (L2) DHan (L2) DrSeegs (L2) Faith1 (L2) Finemlucis (L2) Gabriela (L2) Gardann (L2) GuuelaD (L2) Hafay (L2) Itos (L2) Kahlid (L2) Lewan (L2) LilDestine (L2) Loadrie (L2) Miley16 (L2) MkaliMitinis3 (L2) Netyap (L2) Nicholasp3 (L2) Rossetti (L2) Rumpelstiltskin (L2) Sarshaun (L2) Soap141 (L2) SoJulia (L2) Tourach (L2) Underpass (L2) Vetrix (L2) Wigglewiggle (L2) Wilder (L2) Winky (L2) Zakai (L2) ZhongYanYuan (L2) Bellis (L3) BourbonZero (L3) Clautastrophe (L3) DuncansLeg (L3) Ellson (L3) Finnry (L3) Jobypre (L3) Jubie (L3) Kingsolomon (L3) KirDoubleO7 (L3) Krypton555 (L3) LiyuLake (L3) Lolly9 (L3) Lumos (L3) MiniLon (L3) MiniMac (L3) Moostard (L3) MsGreen (L3) Nicholas (L3) Red305 (L3) Samty (L3) Snenia (L3) TriFive (L3) Whirlwind (L3) BrainDrainer (L4) Bromden (L4) Chaser (L4) Douge (L4) Douzhi (L4) DyoEdafos (L4) FarmResident (L4) Kropertea (L4) PYPDinur (L4) Quby (L4) Sheng711 (L4) Aegeus (L5) Baudelaire (L5)
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Clear Plaque.Sometimes turbid.
Morphotype	Siphoviridae
Number of Genes	129
Number of tRNAs	11
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View GuuelaD_1 GuuelaD_2 (terminase small subunit) GuuelaD_3 GuuelaD_4 (terminase large subunit) GuuelaD_5 (portal protein) GuuelaD_6 (MuF-like minor capsid protein) GuuelaD_7 (scaffolding protein) GuuelaD_8 (major capsid protein) GuuelaD_9 (head-to-tail adaptor) GuuelaD_10 (head-to-tail stopper) GuuelaD_11 GuuelaD_12 (tail terminator) GuuelaD_13 (major tail protein) GuuelaD_14 (tail assembly chaperone) GuuelaD_15 (tail assembly chaperone) GuuelaD_16 (tape measure protein) GuuelaD_17 (minor tail protein) GuuelaD_18 (minor tail protein) GuuelaD_19 (minor tail protein) GuuelaD_20 (minor tail protein) GuuelaD_21 (minor tail protein) GuuelaD_22 (minor tail protein) GuuelaD_23 GuuelaD_24 (minor tail protein) GuuelaD_25 GuuelaD_26 GuuelaD_27 (lysin A) GuuelaD_28 (lysin B) GuuelaD_29 GuuelaD_30 GuuelaD_31 GuuelaD_32 (NrdH-like glutaredoxin) GuuelaD_33 GuuelaD_34 GuuelaD_35 (nucleotidyltransferase) GuuelaD_36 (Lsr2-like DNA bridging protein) GuuelaD_37 GuuelaD_38 (tyrosine integrase) GuuelaD_39 GuuelaD_40 (immunity repressor) GuuelaD_41 (Cro protein) GuuelaD_42 (excise) GuuelaD_43 GuuelaD_44 GuuelaD_45 GuuelaD_46 (esterase) GuuelaD_47 GuuelaD_48 GuuelaD_49 GuuelaD_50 GuuelaD_51 (band-7-like membrane protein) GuuelaD_52 GuuelaD_53 GuuelaD_54 (Ro-like RNA binding protein) GuuelaD_55 GuuelaD_56 GuuelaD_57 GuuelaD_58 GuuelaD_59 GuuelaD_60 GuuelaD_61 GuuelaD_62 (ERF family ssDNA binding protein) GuuelaD_63 GuuelaD_64 GuuelaD_65 GuuelaD_66 (HNH endonuclease) GuuelaD_67 GuuelaD_68 (DnaB-like dsDNA helicase) GuuelaD_69 (WhiB family transcription factor) GuuelaD_70 GuuelaD_71 GuuelaD_72 (phosphoribosyl pyrophosphate transferase) GuuelaD_73 (nicotinate ribosyltransferase) GuuelaD_74 (polynucleotide kinase) GuuelaD_75 GuuelaD_76 GuuelaD_77 GuuelaD_78 (RusA-like resolvase) GuuelaD_79 (WhiB family transcription factor) GuuelaD_80 (WhiB family transcription factor) GuuelaD_81 GuuelaD_82 GuuelaD_83 (ssDNA-binding protein) GuuelaD_84 GuuelaD_85 (RNA ligase) GuuelaD_86 GuuelaD_87 (ClpP-like protease) GuuelaD_88 GuuelaD_89 GuuelaD_90 GuuelaD_91 GuuelaD_92 (HNH endonuclease) GuuelaD_93 GuuelaD_94 GuuelaD_95 (DnaQ-like DNA polymerase III subunit) GuuelaD_96 GuuelaD_97 (HNH endonuclease) GuuelaD_98 GuuelaD_99 GuuelaD_100 GuuelaD_101 — tRNA GuuelaD_102 GuuelaD_103 — tRNA GuuelaD_104 — tRNA GuuelaD_105 GuuelaD_106 GuuelaD_107 GuuelaD_108 — tRNA GuuelaD_109 — tRNA GuuelaD_110 — tRNA GuuelaD_111 — tRNA GuuelaD_112 — tRNA GuuelaD_113 GuuelaD_114 GuuelaD_115 — tRNA GuuelaD_116 GuuelaD_117 GuuelaD_118 GuuelaD_119 — tRNA GuuelaD_120 GuuelaD_121 — tRNA GuuelaD_122 GuuelaD_123 GuuelaD_124 GuuelaD_125 GuuelaD_126 GuuelaD_127 GuuelaD_128 GuuelaD_129 GuuelaD_130 GuuelaD_131 GuuelaD_132 GuuelaD_133 GuuelaD_134 GuuelaD_135 GuuelaD_136 (AAA-ATPase) GuuelaD_137 GuuelaD_138 GuuelaD_139 GuuelaD_140
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MF324910
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	1.8X10E9 pfu/ml
Pitt Freezer Box#	93
Pitt Freezer Box Grid#	B2
Available Files
Plaque Picture	Download
EM Picture	Download
Final DNAMaster File	Download

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