| Detailed Information for Phage JCole | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | L Tesche R Avena R Manjila V Ginjupally |
| Year Found | 2022 |
| Location Found | Pittsburgh, PA United States of America |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.43934 N, 79.954129 W Map |
| Discovery Notes | Phage was found in a borough of Pittsburgh called South Oakland in an alley underneath a trash can by L Tesche. Phage JCole was on the same purification plate as phage Turkey. The plaque chosen was of a different morphology than phage Turkey, however, restriction digest supports that these phage are duplicates and no DNA was isolated for phage Turkey. DNA extraction and restriction digest was performed by A. Morita, J. Noh, J. Ruit, J. Wang, |
| Naming Notes | We chose JCole as the name of our phage. The name of our phage came from a well-known artist who released the song "Middle Child". During the process of purifying of our phage, we viewed the difficulties we experienced as comparable as the behavior of a middle child. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 5, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 3710 |
| Genome length (bp) | 45137 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TACCAGGGGGA |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CZ |
| Subcluster | CZ2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewBunnybear (None) Lton (None) Ohgeesy (None) Pepperoni (None) Agueybana (CZ1) AlumE (CZ1) Antonio (CZ1) BatStarr (CZ1) Bialota (CZ1) Bosnia (CZ1) BoyNamedSue (CZ1) Eudoria (CZ1) Eviarto (CZ1) Herod (CZ1) Hugley (CZ1) Hyzer (CZ1) Kita (CZ1) Madeline (CZ1) Manasvini (CZ1) Maridalia (CZ1) Neobush (CZ1) Nymphadora (CZ1) Polly (CZ1) Suscepit (CZ1) Tayonia (CZ1) ThankyouJordi (CZ1) TimTam (CZ1) Trumpet (CZ1) WelcomeAyanna (CZ1) Zameen (CZ1) Zirinka (CZ1) Attis (CZ2) Bjanes7 (CZ2) Clap (CZ2) Cynthia (CZ2) Ebert (CZ2) EdnaMode (CZ2) GemG (CZ2) Gizermo (CZ2) Haley23 (CZ2) JCole (CZ2) Lamberg (CZ2) LordFarquaad (CZ2) Matteo (CZ2) Mocha12 (CZ2) Nettuno (CZ2) Pickett (CZ2) Sahara (CZ2) Savage (CZ2) SoilAssassin (CZ2) Sproutie (CZ2) TuertoX (CZ2) Vasanti (CZ2) Whiteclaw (CZ2) Yeet412 (CZ2) BaxterFox (CZ3) PantheRoc (CZ3) Yeezy (CZ3) Adora (CZ4) Annalisa (CZ4) Beenie (CZ4) Clark (CZ4) DobbysSock (CZ4) Dolores (CZ4) Dorito (CZ4) Easley (CZ4) Hortense (CZ4) Howe (CZ4) Mcklovin (CZ4) MichaelScott (CZ4) Oregano (CZ4) PhriskyACE (CZ4) Powerball (CZ4) Samman98 (CZ4) Sekhmet (CZ4) Shlim410 (CZ4) Suerte (CZ4) Thimann (CZ4) Twinkle (CZ4) WinkNick (CZ4) Denise (CZ5) Faith5x5 (CZ6) Moosehead (CZ6) OneDirection (CZ6) Sidious (CZ7) RavenCo17 (CZ8) |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | Overdue (Expected completion by 9/2/2024) |
| Plaque Notes | Phage JCole's plaque was hazy and cloudy. Additionally, it was bigger than the plaque of phage Turkey. |
| Morphotype | Siphoviridae |
| Number of Genes | 72 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewJCole_1 JCole_2 JCole_3 JCole_4 JCole_5 JCole_6 JCole_7 JCole_8 JCole_9 JCole_10 JCole_11 JCole_12 JCole_13 JCole_14 JCole_15 JCole_16 JCole_17 JCole_18 JCole_19 JCole_20 JCole_21 JCole_22 JCole_23 JCole_24 JCole_25 JCole_26 JCole_27 JCole_28 JCole_29 JCole_30 JCole_31 JCole_32 JCole_33 JCole_34 JCole_35 JCole_36 JCole_37 JCole_38 — tRNA JCole_39 JCole_40 JCole_41 JCole_42 JCole_43 JCole_44 JCole_45 JCole_46 JCole_47 JCole_48 JCole_49 JCole_50 JCole_51 JCole_52 JCole_53 JCole_54 JCole_55 JCole_56 JCole_57 JCole_58 JCole_59 JCole_60 JCole_61 JCole_62 JCole_63 JCole_64 JCole_65 JCole_66 JCole_67 JCole_68 JCole_69 JCole_70 JCole_71 JCole_72 JCole_73 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 159 |
| Pitt Freezer Box Grid# | F8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
