| Detailed Information for Phage MiniLon |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Lonika Oodhrajh |
| Year Found | 2018 |
| Location Found | Durban, South Africa |
| Finding Institution | University of Kwazulu-Natal |
| Program | Mycobacterial Genetics Course, Durban, South Africa |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 29.816889 S, 30.941889 E Map |
| Discovery Notes | This soil sample had been enriched for 5 days, plated out on the 5th day, incubated for 2 days and then showed plaques on the morning of the 3rd day. |
| Naming Notes | This phage formed really tiny plaques, hence the use of Mini in its name and Lon comes from the founder of this phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 22, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1309 |
| Genome length (bp) | 75793 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Kwazulu-Natal |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are extremely small. Their size is not uniform, however, all plaques are circular. |
| Morphotype | Siphoviridae |
| Number of Genes | 133 |
| Number of tRNAs | 11 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT889383 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
