| Detailed Information for Phage Neobush |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Aakriti Neopaney (Adopted by Madeline Bush) |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.340556 N, 79.970278 W Map |
| Discovery Notes | My soil sample was collected from the flowerbed in the backyard of my house. My house is located in Baldwin, PA. I collected my soil sample on Monday, September 23rd 2019 at 7:48AM. The soil was slightly moist and covered in dead roots. The soil was collected directly from the surface. It was gloomy outside with an ambient temperature of 67º F. There were a few plants behind the area in which I collected the soil from. |
| Naming Notes | Phage Neobush was isolated and discovered by Neopaney and adopted by Bush. The parents of the phage decided to combine their last names for their special child that was found on the sixth isolation trial. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 24, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1775 |
| Genome length (bp) | 50906 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Washington State University |
| Annotation Status | In GenBank |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW924650 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
114 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
