| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Reagan C., Zoe H., Clarissa M., & Camila A. |
| Year Found | 2025 |
| Location Found | Longview, TX United States |
| Finding Institution | LeTourneau University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 32.46458 N, 94.72705 W Map |
| Discovery Notes | At 3:23 PM CDT on August 21, 2025, the soil sample was collected from around a tree behind The Glaske Center for Engineering, Science, and Technology at LeTourneau University. The hole was approximately 6.3 cm deep, and the soil was very moist and squishy. The weather was sunny with a slightly cloudy sky and ambient temperature of 28.3℃. The soil sample was collected by Breeanna A. Brege and Naveyah M. Raukar, who kindly donated a plate with plaques to us for virus isolation and purification. |
| Naming Notes | The name “OverPar” ties into golf, where scoring over par means you’ve taken more strokes than ideal, and things didn’t go smoothly. It’s a playful nod to our project, which faced plenty of setbacks and obstacles along the way. Just like a tough hole on the course, we kept hitting challenges, so the name reflects both the difficulty and a bit of humor about our less-than-perfect “round.” |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 1055 |
| Genome length (bp) | 69993 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 58.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | LeTourneau University |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Cloudy/turbid plaques, measured a total of 10 plaques which resulted in an average a diameter of 0.168 mm and a range of 0.10-0.28 mm; n = 10. |
| Number of Genes | 118 |
| Number of tRNAs | 8 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
2.1 x 10^9 PFU/mL |
| Available Files |
| Plaque Picture | Download |
