| Detailed Information for Phage Rose5 |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Joel Winthrope |
| Year Found | 2019 |
| Location Found | Corpus Christi, TX United States of America |
| Finding Institution | Del Mar College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 29°C |
| GPS Coordinates | 27.65 N, 97.41 W Map |
| Discovery Notes | Gathered from Botanical gardens.
Soil sample was taken next to cinco de mayo roses.
Soil temperature was 29.4 degrees celsius.
Ambient temperature was 29.8 degrees celsius.
Ambient humidity was 100%; it had rained approximately an hour before collection.
Closest body of water is the Oso river. |
| Naming Notes | named because sample was taken from a rose garden. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 14, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 643 |
| Genome length (bp) | 73949 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGAACGGCC |
| GC Content | 58.7% |
| Sequencing Notes | The sample that was sequenced seemed to be contaminated with a low level (~5%) of phage Nanosmite. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Washington State University |
| Annotation Status | In GenBank |
| Plaque Notes | Avg. plaque diameter is 1mm
plaques are very clear
Lytic life cycle
seems to be very aggressive |
| Morphotype | Siphoviridae |
| Number of Genes | 123 |
| Number of tRNAs | 10 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW822149 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
101 |
| Pitt Freezer Box Grid# |
A1 |
| Available Files |
| GenBank File for Phamerator | Download |