Mycobacterium phage Rumpelstiltskin
Rumpelstiltskin Plaque Picture
Rumpelstiltskin EM Picture
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Detailed Information for Phage Rumpelstiltskin
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found BySinead Gilmore and Dr. Louise Temple
Year Found2009
Location FoundUnavailable
Finding InstitutionJames Madison University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.7456 N, 74.4975 W Map
Discovery NotesThis is an area of residences of large acreage, but not really farms.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 1, 2011
Genome length (bp)69279
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCGATCAGCC
GC Content58.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterL
SubclusterL2
Cluster Life CycleTemperate
Other Cluster Members

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Acquire49 (L1)

Appletree2 (L1)

AvadaKedavra (L1)

Calm (L1)

CicholasNage (L1)

DirkDirk (L1)

Enceladus (L1)

Halena (L1)

JoeDirt (L1)

LeBron (L1)

MAckerman (L1)

OhShagHennessy (L1)

Rose5 (L1)

Silverleaf (L1)

Tyson (L1)

UPIE (L1)

Wamburgrxpress (L1)

Wyatt2 (L1)

Zaria (L1)

Archie (L2)

Baoshan (L2)

Bazzle (L2)

BigCheese (L2)

BobsGarage (L2)

Breezona (L2)

Claus (L2)

Crossroads (L2)

DrSeegs (L2)

Faith1 (L2)

Finemlucis (L2)

Gabriela (L2)

Gardann (L2)

GuuelaD (L2)

Hafay (L2)

Itos (L2)

Kahlid (L2)

Lewan (L2)

LilDestine (L2)

Loadrie (L2)

Miley16 (L2)

MkaliMitinis3 (L2)

Netyap (L2)

Nicholasp3 (L2)

Rumpelstiltskin (L2)

Tourach (L2)

Vetrix (L2)

Wigglewiggle (L2)

Wilder (L2)

Winky (L2)

Zakai (L2)

ZhongYanYuan (L2)

Bellis (L3)

Clautastrophe (L3)

DuncansLeg (L3)

Ellson (L3)

Finnry (L3)

Jobypre (L3)

Jubie (L3)

Kingsolomon (L3)

Krypton555 (L3)

Lolly9 (L3)

Lumos (L3)

MiniLon (L3)

MiniMac (L3)

Moostard (L3)

MsGreen (L3)

Nicholas (L3)

Samty (L3)

Snenia (L3)

Whirlwind (L3)

Bromden (L4)

Chaser (L4)

Douge (L4)

DyoEdafos (L4)

Aegeus (L5)

Baudelaire (L5)
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesQuite small, clear plaques.
Incubated overnight/24hrs at 37°C
MorphotypeSiphoviridae
Number of Genes106
Number of tRNAs1
Has been Phamerated?Yes
Gene List

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Rumpelstiltskin_1

Rumpelstiltskin_2

Rumpelstiltskin_3

Rumpelstiltskin_4
(terminase large subunit)

Rumpelstiltskin_5
(portal protein)

Rumpelstiltskin_6
(MuF-like minor capsid protein)

Rumpelstiltskin_7

Rumpelstiltskin_8
(major capsid protein)

Rumpelstiltskin_9
(head-to-tail adaptor)

Rumpelstiltskin_10
(head-to-tail stopper)

Rumpelstiltskin_11

Rumpelstiltskin_12
(tail terminator)

Rumpelstiltskin_13
(major tail protein)

Rumpelstiltskin_14
(tail assembly chaperone)

Rumpelstiltskin_15
(tail assembly chaperone)

Rumpelstiltskin_16
(tape measure protein)

Rumpelstiltskin_17
(minor tail protein)

Rumpelstiltskin_18
(minor tail protein)

Rumpelstiltskin_19
(minor tail protein)

Rumpelstiltskin_20
(minor tail protein)

Rumpelstiltskin_21

Rumpelstiltskin_22

Rumpelstiltskin_23

Rumpelstiltskin_24

Rumpelstiltskin_25

Rumpelstiltskin_26

Rumpelstiltskin_27
(lysin A)

Rumpelstiltskin_28
(lysin B)

Rumpelstiltskin_29

Rumpelstiltskin_30

Rumpelstiltskin_31

Rumpelstiltskin_32
(NrdH-like glutaredoxin)

Rumpelstiltskin_33

Rumpelstiltskin_34

Rumpelstiltskin_35
(nucleotidyl transferase)

Rumpelstiltskin_36
(Lsr2-like DNA bridging protein)

Rumpelstiltskin_37

Rumpelstiltskin_38
(tyrosine integrase)

Rumpelstiltskin_39

Rumpelstiltskin_40

Rumpelstiltskin_41

Rumpelstiltskin_42

Rumpelstiltskin_43

Rumpelstiltskin_44

Rumpelstiltskin_45

Rumpelstiltskin_46

Rumpelstiltskin_47
(esterase)

Rumpelstiltskin_48

Rumpelstiltskin_49

Rumpelstiltskin_50

Rumpelstiltskin_51

Rumpelstiltskin_52

Rumpelstiltskin_53

Rumpelstiltskin_54
(Ro-like RNA binding protein)

Rumpelstiltskin_55

Rumpelstiltskin_56

Rumpelstiltskin_57

Rumpelstiltskin_58

Rumpelstiltskin_59

Rumpelstiltskin_60

Rumpelstiltskin_61

Rumpelstiltskin_62
(ssDNA binding protein, ERF family)

Rumpelstiltskin_63

Rumpelstiltskin_64

Rumpelstiltskin_65

Rumpelstiltskin_66
(HNH endonuclease)

Rumpelstiltskin_67

Rumpelstiltskin_68

Rumpelstiltskin_69
(DnaB-like dsDNA helicase)

Rumpelstiltskin_70
(WhiB family transcription factor)

Rumpelstiltskin_71

Rumpelstiltskin_72

Rumpelstiltskin_73
(phosphoribosyl pyrophosphate transferase)

Rumpelstiltskin_74
(nicotinate ribosyltransferase)

Rumpelstiltskin_75
(polynucleotide kinase)

Rumpelstiltskin_76

Rumpelstiltskin_77
(RusA-like resolvase)

Rumpelstiltskin_78
(WhiB family transcription factor)

Rumpelstiltskin_79
(WhiB family transcription factor)

Rumpelstiltskin_80

Rumpelstiltskin_81

Rumpelstiltskin_82
(ssDNA binding protein)

Rumpelstiltskin_83

Rumpelstiltskin_84
(RNA ligase)

Rumpelstiltskin_85

Rumpelstiltskin_86
(ClpP-like protease)

Rumpelstiltskin_87

Rumpelstiltskin_88

Rumpelstiltskin_89

Rumpelstiltskin_90

Rumpelstiltskin_91
(HNH endonuclease)

Rumpelstiltskin_92

Rumpelstiltskin_93

Rumpelstiltskin_94
(DnaQ-like DNA polymerase III subunit)

Rumpelstiltskin_95

Rumpelstiltskin_96

Rumpelstiltskin_97

Rumpelstiltskin_98
(endonuclease VII)

Rumpelstiltskin_99

Rumpelstiltskin_100

Rumpelstiltskin_101

Rumpelstiltskin_103

Rumpelstiltskin_104

Rumpelstiltskin_105

Rumpelstiltskin_106

Rumpelstiltskin_107
(AAA-ATPase)

Rumpelstiltskin_108

Rumpelstiltskin_109

Rumpelstiltskin_110

Rumpelstiltskin_111
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionJN680858
Refseq NumberNC_023732
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.4e7
Pitt Freezer Box# 2
Pitt Freezer Box Grid# H6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload