| Detailed Information for Phage SoilAssassin |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Daniel Biery |
| Year Found | 2015 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.446343 N, 79.963773 W Map |
| Discovery Notes | SoilAssassin was adopted from another scientist who originally isolated it. |
| Mutants |
| Mutants of this Phage |
Click to View
501B
503D
MM550
MM551
MM552
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 13, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 932 |
| Genome length (bp) | 47880 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TACCAGGGGGA |
| GC Content | 66.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ2 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | If the concentration of phage plated it too high, no bacteria will be killed and the plate will appear as though no phage was plated with it. A slightly lower concentration of phage will clear out the plate entirely. |
| Other Cluster Members |
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| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are between 2 to 4 mm. In the center of the plaque, there is a clearing straight through the bacterial lawn. Around the central clearing is a halo that is not cleared down through the lawn. |
| Number of Genes | 74 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU963246 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.43x10^10 |
| Pitt Freezer Box# |
3 |
| Pitt Freezer Box Grid# |
A2 |
| Available Files |
| Plaque Picture | Download |
| Final DNAMaster File | Download |
