Gordonia phage SoilAssassin
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Detailed Information for Phage SoilAssassin
Discovery Information
Isolation HostGordonia terrae 3612
Found ByDaniel Biery
Year Found2015
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature30°C
GPS Coordinates40.446343 N, 79.963773 W Map
Discovery NotesSoilAssassin was adopted from another scientist who originally isolated it.
Mutants
Mutants of this Phage

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501B

503D

MM550

MM551

MM552

Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 13, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage932
Genome length (bp)47880
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTACCAGGGGGA
GC Content66.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ2
Cluster Life CycleTemperate
Lysogeny NotesIf the concentration of phage plated it too high, no bacteria will be killed and the plate will appear as though no phage was plated with it. A slightly lower concentration of phage will clear out the plate entirely.
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques are between 2 to 4 mm. In the center of the plaque, there is a clearing straight through the bacterial lawn. Around the central clearing is a halo that is not cleared down through the lawn.
Number of Genes74
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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SoilAssassin_1
(terminase small subunit)

SoilAssassin_2
(terminase large subunit)

SoilAssassin_3
(portal protein)

SoilAssassin_4
(capsid maturation protease)

SoilAssassin_5
(scaffolding protein)

SoilAssassin_6
(major capsid protein)

SoilAssassin_7

SoilAssassin_8
(head-to-tail adaptor)

SoilAssassin_9

SoilAssassin_10

SoilAssassin_11

SoilAssassin_12
(major tail protein)

SoilAssassin_13
(tail assembly chaperone)

SoilAssassin_14
(tail assembly chaperone)

SoilAssassin_15
(tape measure protein)

SoilAssassin_16
(minor tail protein)

SoilAssassin_17
(minor tail protein)

SoilAssassin_18
(lysin A)

SoilAssassin_19

SoilAssassin_20

SoilAssassin_21

SoilAssassin_22

SoilAssassin_23

SoilAssassin_24
(lysin B)

SoilAssassin_25
(minor tail protein)

SoilAssassin_26

SoilAssassin_27

SoilAssassin_28
(minor tail protein)

SoilAssassin_29

SoilAssassin_30

SoilAssassin_31

SoilAssassin_32

SoilAssassin_33
(lipoprotein)

SoilAssassin_34
(integrase)

SoilAssassin_35

SoilAssassin_36
(antitoxin)

SoilAssassin_37

SoilAssassin_38

SoilAssassin_39
(immunity repressor)

SoilAssassin_40
(helix-turn-helix DNA binding domain protein)

SoilAssassin_41

SoilAssassin_42

SoilAssassin_43
(antirepressor)

SoilAssassin_44

SoilAssassin_45

SoilAssassin_46

SoilAssassin_47

SoilAssassin_48

SoilAssassin_49

SoilAssassin_50

SoilAssassin_51
(RecE-like exonuclease)

SoilAssassin_52
(RecT-like ssDNA binding protein)

SoilAssassin_53

SoilAssassin_54

SoilAssassin_55

SoilAssassin_56

SoilAssassin_57

SoilAssassin_58

SoilAssassin_59

SoilAssassin_60

SoilAssassin_61
(HTH DNA-binding protein)

SoilAssassin_62

SoilAssassin_63

SoilAssassin_64
(HNH endonuclease)

SoilAssassin_65

SoilAssassin_66

SoilAssassin_67

SoilAssassin_68

SoilAssassin_69
(helix-turn-helix DNA binding domain protein)

SoilAssassin_70

SoilAssassin_71

SoilAssassin_72

SoilAssassin_73

SoilAssassin_74
(HNH endonuclease)

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKU963246
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.43x10^10
Pitt Freezer Box# 3
Pitt Freezer Box Grid# A2
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload