Gordonia phage Sproutie
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| Detailed Information for Phage Sproutie | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Desiree Paige Rose |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.44222 N, 79.95611 W Map |
| Discovery Notes | The soil sample was collected entirely in a clean plastic bag at approximately 10:30 on Monday January 23, 2017 with a temperature of 46°F. The soil was collected from a flower pot, and the soil was very moist, full of organic matter, and collected approximately 2 inches below the surface. |
| Naming Notes | I named this phage with the help of my mother by considering the location of its origin. The soil sample was collected from a flower pot area, and plants sprout out of the ground, so Sproutie did too. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 16, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1967 |
| Genome length (bp) | 46221 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TACCAGGGGGA |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CZ |
| Subcluster | CZ2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Winthrop University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque formed was small,round, and clear in color. |
| Number of Genes | 75 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK279911 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 56 |
| Pitt Freezer Box Grid# | D11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
