| Detailed Information for Phage Tayonia |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Former names | TayoniaSII |
| Found By | Taylor Campbell |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.4425 N, 79.9225 W Map |
| Discovery Notes | The soil was collected in a flower bed on the Litchfield Towers Patio on campus at the University of Pittsburgh at 2:36 pm on 9/10/18. It was cloudy and raining at the time of collection. The soil was dark and moist. The collection site was mainly fresh soil with flowers and plants growing in the flower bed. |
| Naming Notes | The name is my own name mixed with the host and an initial of a last name with "II" meaning junior or the second. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 547 |
| Genome length (bp) | 50320 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | After performing a spot test within the lysogeny protocol, mesas began forming on the first and second spots. Further testing must be completed to confirm lysogens. |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The morphology for this phage seems to show up as two different clearings one small with a slightly smaller one also present. This pattern remained consistent through three rounds of purification. |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK875796 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
10x10^11 |
| Pitt Freezer Box# |
91 |
| Pitt Freezer Box Grid# |
F6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
