| Detailed Information for Phage TriFive |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nytiyanna Robinson |
| Year Found | 2024 |
| Location Found | Conway, SC USA |
| Finding Institution | Coastal Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.79713 N, 79.01017 W Map |
| Discovery Notes | The location of the isolation was outside of Swain Hall at Coastal Carolina University on the side closest to Douglas Hall. Conditions were dry, but the sample contained moisture. The sample was also collected roughly 5-7 inches into the ground. |
| Naming Notes | The origin of TriFive comes from it being the fifth attempt at isolating a phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2760 |
| Genome length (bp) | 75732 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | TCGATCAGCC |
| GC Content | 59.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | L |
| Subcluster | L3 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Coastal Carolina University |
| Annotation Status | In GenBank |
| Plaque Notes | TriFive makes small and large plaques that were circular/oval-like. They were relatively uniform and slightly cloudy. |
| Morphotype | Siphoviridae |
| Number of Genes | 128 |
| Number of tRNAs | 10 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PX512281 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
197 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
