| Detailed Information for Phage Trumpet |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Caitlyn Kline |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.443694 N, 79.954806 W Map |
| Discovery Notes | For every protocol after purification I used a modified protocol with 125uL of Gordonia terrae cells instead of 250 and also diluted the top agar increase the plaque size of Trumpet. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 24, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1262 |
| Genome length (bp) | 50338 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCTGGGGA |
| GC Content | 66.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CZ |
| Subcluster | CZ1 |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | Trumpet is Virulent. |
| Other Cluster Members |
|
| Annotating Institution | Washington State University |
| Annotation Status | In GenBank |
| Plaque Notes | Trumpet is growing in PYCa growth medium with Gordonia terrae host cells and incubated at 30*C for 2 days. Plaques are symmetrical and lytic with a diameter with less than 1mm in diameter. |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW924648 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
115 |
| Pitt Freezer Box Grid# |
D10 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
